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Differentially expressed genes in a flock of Chinese local-breed chickens infected with a subgroup J avian leukosis virus using suppression subtractive hybridization Genet. Mol. Biol.
Zhao,Guiping; Zheng,Maiqing; Chen,Jilan; Wen,Jie; Wu,Chunmei; Li,Wenjuan; Liu,Libo; Zhang,Yuan.
Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian leukosis virus subgroup J; Chicken; Transferring gene; MHC genes; Hemoglobin gene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100009
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Histomorphometric Characterization of Forestomach of Yak (Bos grunniens) in the Qinghai-Tibetan Plateau International Journal of Morphology
Wang,Jinbao; Li,Haiyan; Zhang,Lu; Zhang,Yuan; Yue,Mingxia; Shao,Baoping; Wang,Jianlin.
Six forestomachs of yaks (Bos grunniens) were studied with gross dissection and histological methods. It was found that the forestomach of yak consisted of the following three parts, rumen, reticulum and omasum, which were composed of the mucosa, submucosa, muscularis and serosa. In addition, the mucosal epithelium was covered with stratified squamous epithelium, with part of keratinized the shallow cells. Rumen, the mucosa of which formed ligulate papillae varying in size and shape, was no muscularis mucosa. Reticulum, consisted of a surface epithelium that invaginated to various extent into the lamina propria, formed various folds in shape, namely, grid-like small rooms. Furthermore, there are many secondary folds densely covered with keratinized...
Tipo: Journal article Palavras-chave: Bos grunniens; Forestomach; Histomorphometric; Qinghai-Tibetan Plateau.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300021
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