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	Provedor de dados BJMBR (3) Autor Zhao,Y. (3) Fang,M. (1) Hu,Y. (1) Jiang,L.-Q. (1) Jin,A. (1) Lan,Y.-J. (1) Liao,Q. (1) Liu,C. (1) Liu,X. (1) Wang,H. (1) Wen,Z. (1) You,L. (1) Zeng,P. (1) Zhang,X.-Q. (1) Zhang,Y. (1) Zhou,J. (1) Zhou,L. (1) Mais... Palavra-chave Acetylate (1) Apoptosis (1) Children (1) Drug testing (1) Glyceraldehyde-3-phosphate dehydrogenase (1) Homocysteine (1) Obstructive sleep apnea-hypopnea syndrome (1) P300/CBP (1) Pancreatic cancer (1) Polysomnography (1) Spheroid (1) Surgery (1) Three-dimensional culture (1) Tonsil (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/report (1) Ano 2017 (1) 2016 (1) 2013 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay BJMBR Wen,Z.; Liao,Q.; Hu,Y.; You,L.; Zhou,L.; Zhao,Y.. Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Compared to two-dimensional culture of cell monolayer, three-dimensional (3-D) models more closely mimic native tissues, since the tumor microenvironment established in 3-D models often plays a significant role in cancer progression and cellular responses to the drugs. Accumulating evidence has highlighted the benefits of 3-D in vitro models of various cancers. In the present study, we have developed a spheroid-based, 3-D culture of pancreatic cancer cell lines MIAPaCa-2 and PANC-1 for pancreatic drug... Tipo: Info:eu-repo/semantics/article Palavras-chave: Spheroid; Three-dimensional culture; Pancreatic cancer; Drug testing. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700634 Homocysteine induces glyceraldehyde-3-phosphate dehydrogenase acetylation and apoptosis in the neuroblastoma cell line Neuro2a BJMBR Fang,M.; Jin,A.; Zhao,Y.; Liu,X.. High plasma levels of homocysteine (Hcy) promote the progression of neurodegenerative diseases. However, the mechanism by which Hcy mediates neurotoxicity has not been elucidated. We observed that upon incubation with Hcy, the viability of a neuroblastoma cell line Neuro2a declined in a dose-dependent manner, and apoptosis was induced within 48 h. The median effective concentration (EC50) of Hcy was approximately 5 mM. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) nuclear translocation and acylation has been implicated in the regulation of apoptosis. We found that nuclear translocation and acetylation of GAPDH increased in the presence of 5 mM Hcy and that higher levels of acetyltransferase p300/CBP were detected in Neuro2a cells. These findings... Tipo: Info:eu-repo/semantics/article Palavras-chave: Homocysteine; Glyceraldehyde-3-phosphate dehydrogenase; Apoptosis; Acetylate; P300/CBP. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200606 Efficacy of minimally invasive tonsil surgery for treatment of obstructive sleep apnea-hypopnea syndrome in children BJMBR Zhang,X.-Q.; Wang,H.; Zhou,J.; Zeng,P.; Zhao,Y.; Zhang,Y.; Liu,C.; Jiang,L.-Q.; Lan,Y.-J.. This study aimed to investigate the efficacy of minimally invasive tonsil surgery for the treatment of obstructive sleep apnea-hypopnea syndrome (OSAHS) in children. Tonsil ablation or turbinate reduction was performed on 49 pediatric patients with OSAHS by minimally invasive tonsil surgery. In order to evaluate the efficacy of surgery, a comparison was conducted between pre-operation and post-operation data in terms of the symptoms, signs and polysomnography test. Total effectiveness rate of the surgery was 83.7%. Subgroup analysis was also performed based on the severity of their conditions: mild, moderate, and severe groups had an effectiveness rate of 90.0, 88.9, and 66.7%, respectively (Hc=6.665, P<0.05). Postoperatively, the apnea-hypopnea index,... Tipo: Info:eu-repo/semantics/report Palavras-chave: Tonsil; Children; Obstructive sleep apnea-hypopnea syndrome; Polysomnography; Surgery. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500703 Registros recuperados: 3 Primeira ... 1 ... Última

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