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| Zhang,Xiao-lin; Jiang,An-min; Ma,Zhong-you; Xiong,You-yi; Dou,Jin-feng; Zhou,Guo-liang; Qin,Mei-song; Wang,Jian-fei. |
| This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus expression system. To determine whether the bombyxin signal peptide resulted in secretory expression of recombinant UreB (rUreB) and to determine the secretory efficiency, we tested the secretory expression level of rUreB in Bm5 cells using ELISA. To further investigate whether secretory expression affected cell viability, cells were evaluated using 0.4% trypan blue staining, and Bacmid/BmNPV/UreB without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Baculovirus expression system; Bombyx mori; Signal peptide; Helicobacter pylori urease subunit B; Secretory expression. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300319 |
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