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	Provedor de dados BABT (4) BJM (2) Autor Zia,Muhammad Anjum (6) Abbas,Rao Zahid (3) Iftikhar,Tehreema (2) Rasul,Samreen (2) Ain,Qurat-ul (1) Arshad,Arooj (1) Asghar,Muhammad (1) Haq,Ikram ul (1) Iqbal,Zafar (1) Joyia,Faiz Ahmad (1) Khan,Muhammad Nisar (1) Niaz,Mubashir (1) Rahman,Khalil-ur (1) Riaz,Ayesha (1) Umbreen,Huma (1) Zafar,Muhammad Arif (1) Mais... Palavra-chave Glucose oxidase (2) A. niger (1) Antibodies (1) Antimicrobial (1) Aspergillus niger (1) Coccidiostat (1) Curcuma longa (1) Eimeria tenella (1) Ethyl methanesulfonate (1) Extracellular lipases (1) Fermentation (1) Fermentor (1) Glucose aerodehydogenase (1) Kinetics (1) Mutagenesis (1) Penicillium notatum (1) Production (1) Purification (1) Rabbits (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Ano 2019 (1) 2013 (2) 2012 (1) 2010 (2) País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Production of rabbit antibodies against purified Glucose oxidase BABT Zia,Muhammad Anjum; Ain,Qurat-ul; Iftikhar,Tehreema; Abbas,Rao Zahid; Rahman,Khalil-ur. Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion exchange column (DEAE-Cellulose) resulted into 22.53 U mg-1 specific activity and 10.27 fold purification. This was applied to sephadex G-200 column for gel filtration chromatography. It was observed that enzyme achieved 59.37 U mg-1of specific activity with 27.08 fold purity and 64.36% recovery. Purified glucose oxidase was injected into rabbits through intravenous route, to raise the glucose oxidase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; A. niger; Antibodies; Rabbits. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100008 Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum BABT Zia,Muhammad Anjum; Riaz,Ayesha; Rasul,Samreen; Abbas,Rao Zahid. This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-200 size exclusion chromatography. The crude enzyme extracts of A. niger and P. notatum showed 2.32 and 5.53 U mg-1 specific activities, respectively, which after desalting was 15.52 and 12.05 U mg-1, and after ion exchange and gel filtration chromatography was 29.09 - 62 and 25.72 - 59.37 U mg-1 for A. niger and P. notatum, respectively. The antimicrobial activity was determined by disc diffusion... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose oxidase; Antimicrobial; Aspergillus niger; Penicillium notatum. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600010 Anticoccidial activity of Curcuma longa L. in broilers BABT Abbas,Rao Zahid; Iqbal,Zafar; Khan,Muhammad Nisar; Zafar,Muhammad Arif; Zia,Muhammad Anjum. Comparative efficacy of turmeric (Curcuma longa L.) crude powder and salinomycin sodium on the occurrence of coccidiosis and growth performance of broiler was evaluated. A total of 90, day-old chicks were randomly divided into six groups. From first day onward, ration was supplemented with 1, 2 and 3 % turmeric powder in groups 2, 3 and 4, respectively, group 1 received salinomycin sodium @ 12 g 50 kg-1 feed while groups 5 and 6 were kept as infected un-medicated and uninfected un-medicated controls. First five groups were infected with Eimeria tenella sporulated oocysts @ 1,00,000/chick at the age of 20 days. Body weight gain, feed consumption, feed conversion ratio were investigated throughout the experimental period, and bloody diarrhea and oocysts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Curcuma longa; Coccidiostat; Eimeria tenella. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100008 Enhanced Production Of Streptokinase By Chemical Mutagenesis Of Streptococcus agalactiae EBL-20 BABT Arshad,Arooj; Zia,Muhammad Anjum; Asghar,Muhammad; Joyia,Faiz Ahmad. Abstract Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases. The current study focused on the enhanced production of SK by inducing mutation in Streptococcus agalactiae EBL-20 and optimization of medium components and culture conditions for the maximum growth of mutant derived strain. S. agalactiae EBL-32 was selected as a potent mutant after exposure of S. agalactiae EBL-20 to EMS for 180 minutes. SK activity obtained from mutant derived strain was found to be 1.6 fold higher as compared to the activity achieved by wild strain. Nutritional requirements of the mutated strain were optimized by single factor analysis method suggesting glucose as the optimum carbon source; yeast extract and peptone as a suitable nitrogen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethyl methanesulfonate; Fermentation; Response surface methodology; Streptokinase. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100200 Kinetic characterization of glucose aerodehydrogenase from Aspergillus niger EMS-150-F after optimizing the dose of mutagen for enhanced production of enzyme BJM Umbreen,Huma; Zia,Muhammad Anjum; Rasul,Samreen. In the present study enhanced production of glucose aerodehydrogenase from Aspergillus niger has been achieved after optimizing the dose of chemical mutagen ethyl methane sulfonate (EMS) that has not been reported earlier. Different doses of mutagen were applied and a strain was developed basing upon the best production. The selected strain Aspergillus niger EMS-150-F was optimized for nutrient requirements in order to produce enzyme through fermentation and the results showed the best yield at 2% corn steep liquor (CSL), 36 hours fermentation time, pH 5, 30°C temperature, 0.3% KH2PO4, 0.3% urea and 0.06% CaCO3. The enzyme was then purified and resulted in 57.88 fold purification with 52.12% recovery. On kinetic characterization, the enzyme showed optimum... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose aerodehydogenase; Mutagenesis; Production; Purification; Thermal stability. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400012 Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor BJM Iftikhar,Tehreema; Niaz,Mubashir; Zia,Muhammad Anjum; Haq,Ikram ul. The present investigation deals with the kinetics of submerged extracellular lipases fermentation by both wild and mutant strains of Rhizopus oligosporus var. microsporus in a laboratory scale stirred fermentor. Other parameters studied were inoculum size, pH, agitation and rate of aeration. It was found that the growth and lipases production was increased gradually and reached its maximum 9.07± 0.42ª U mL-1 (W) and 42.49 ± 3.91ª U mL-1 (M) after 30h of fermentation for both wild and mutant strain. There is overall increase of 109% (W) and 124% (M) in the production of extracellular lipases as compared to shake flask. Another significant finding of the present study is that the fermentation period is reduced to 30 h in case of wild and 23 h in case of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipases; Rhizopus oligosporus; Fermentor; Kinetics. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400034 Registros recuperados: 6 Primeira ... 1 ... Última

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