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| Ziech,Rosangela Estel; Lampugnani,Camila; Perin,Ana Paula; Sereno,Mallu Jagnow; Sfaciotte,Ricardo Antônio Pilegi; Viana,Cibeli; Soares,Vanessa Mendonça; Pinto,José Paes de Almeida Nogueira; Bersot,Luciano dos Santos. |
| Abstract The aim of this study was to investigate the occurrence of multidrug-resistant, extended spectrum beta-lactamase (ESBL) producing Salmonella spp. isolated from conveyor belts of broiler cutting rooms in Brazilian broiler processing plants. Ninety-eight strains of Salmonella spp. were analyzed. Multidrug resistance was determined by the disk diffusion test and the susceptibility of the isolated bacteria was evaluated against 18 antimicrobials from seven different classes. The double disk diffusion test was used to evaluate ESBL production. Of the 98 strains tested, 84 were multidrug resistant. The highest rates of resistance were against nalidixic acid (95%), tetracycline (91%), and the beta-lactams: ampicillin and cefachlor (45%), followed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial resistance; Salmonella spp.; Food borne pathogens. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100191 |
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| Ziech,Rosangela Estel; Farias,Luana D’Avila; Ardisson-Araújo,Daniel Mendes Pereira; Camargo,Gregório Miguel Ferreira de; Frey,Joachim; Vargas,Agueda Castagna de. |
| ABSTRACT: Clostridium chauvoei toxin A (CctA), neuraminidase (NanA), and flagellin (FliC) proteins contribute to the pathogenicity of Clostridium chauvoei, the causative agent of blackleg in cattle. The aim of this study was to analyze the genetic variability of cctA, nanA, and fliC genes in C. chauvoei isolates from the Rio Grande do Sul and São Paulo state- Brazil, during different sampling periods. The presence of these genes was verified through PCR amplification and partial gene sequencing of 17 strains. Alignment of PCR amplicons combined with bioinformatics analysis was used in an attempt to study the variability across C. chauvoei solates. The similarity among the partial sequences of cctA and nanA genes was 100%. The sequencing of fliC revealed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blackleg; Clostridium chauvoei toxin A; Neuraminidase; Flagellin. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500452 |
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| Ziech,Rosangela Estel; Gressler,Leticia Trevisan; Frey,Joachim; Vargas,Agueda Castagna de. |
| ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiologic agent is the anaerobic bacterium Clostridium chauvoei. In recent years, the major virulence factors of C. chauvoei have been discovered and described. However, the pathogenesis of blackleg in cattle, and in particular, the movement of the pathogen from the point of entry to the affected tissues is not yet fully elucidated. Disease control is based on appropriate management and vaccination. This review summarizes the latest research findings that contribute toward the understanding of the disease in cattle, provide a foundation to preventive strategies, and identify future research needs. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium chauvoei; Sudden death; Myonecrosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500454 |
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| Balzan,Cláudia; Ziech,Rosangela Estel; Gressler,Letícia Trevisan; Vargas,Agueda Palmira Castagna de. |
| ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus subsp. venerealis. In countries with large cattle herds, such as Brazil, where the use of natural breeding as a reproductive strategy is a common practice, BGC is considered an important cause of reproductive failure and economic losses. In these cases, the bull is the asymptomatic carrier of the bacterium and the infected females can have infertility and even abortions. The techniques for the diagnosis of C. fetus are isolation in culture medium and identification by biochemical tests, immunofluorescence, immunoenzymatic assays and molecular techniques. Disease control is based on vaccination with bacterins. This review described the epidemiology,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter fetus subsp. venerealis; Infertility; Venereal disease; Beef cattle.. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300451 |
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| Ziech,Rosangela Estel; Machado,Gustavo; Kirinus,Jackeline Karsten; Libardoni,Felipe; Kessler,Julcemar Dias; Pötter,Luciana; Vargas,Agueda Castagna de. |
| A campilobacteriose genital bovina (CGB) é uma doença infectocontagiosa causada por Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, além de aumentar o intervalo entre partos. A ocorrência de CGB entre rebanhos no Brasil tem variado muito entre as diferentes regiões. Com isso, o objetivo deste trabalho foi identificar, por meio da reação em cadeia da polimerase (PCR), a ocorrência de amostras positivas para C. fetus, oriundas de bovinos, no período de 1999 a 2010, no Rio Grande do Sul, e analisar a positividade em machos e fêmeas. Foram utilizadas 816 amostras procedentes de 37 municípios, localizados predominantemente nas mesorregiões sudoeste e centro ocidental rio-grandense, das quais 480 aspirados prepuciais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Doença venérea; Campilobacteriose genital bovina; Diagnóstico; Frequência. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000100023 |
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