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Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing 55
d'Azevedo,Pedro A.; Gonçalves,Ana Lúcia S.; Musskopf,Maiara I.; Ramos,Carina G.; Dias,Cícero A. G..
Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67%) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54%) isolates were confirmed as ESBL producers, and 18/107 (17%) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83%) of strains that did not show CAIE. ESBL detection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; Double disk confirmatory test; Cefoxitin susceptibility testing; Screening test.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500006
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MRSA from Santa Catarina State, Southern Brazil: intriguing epidemiological differences compared to other Brazilian regions 55
Silveira,Alessandro C.O.; Cunha,Gabriela R.; Caierão,Juliana; Cordova,Caio M. de; d'Azevedo,Pedro A..
Methicillin-resistant Staphylococcus aureus(MRSA) is one of the most frequently isolated agents in both nosocomial and community settings. It is a constant challenge for antibacterial therapy. Therefore, it becomes essential to understand the epidemiology of MRSA isolates in the institution and/or region to guide empirical therapy. The objective of this study was to evaluate the epidemiological characteristics of MRSA isolates in the state of Santa Catarina, Brazil, and determine if there is a clonal spread. We evaluated 124 clinical isolates of MRSA obtained from various anatomical sites from patients in the state of Santa Catarina in Southern Brazil. The antimicrobial susceptibility profile was evaluated by disk diffusion and minimum inhibitory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial resistance; Vancomycin; Oxacillin.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400384
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