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Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14 69
Moura Leal,Maria de Lourdes; da Silva Gomes Pereira,Denise; Jessouroun,Ellen; Peixoto Gimenes Couto,Maria Antonieta; Pereira Jr,Nei.
Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an...
Tipo: Journal article Palavras-chave: Polysaccharide capsular; Vaccine; Streptococcus pneumoniae.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006
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