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| de-Souza,W.; de-Carvalho,T.U.; de-Melo,E.T.; Soares,C.P.; Coimbra,E.S.; Rosestolato,C.T.; Ferreira,S.R.; Vieira,M.. |
| In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Confocal laser scanning microscopy; Host cell-parasite interaction; Trypanosoma cruzi; Toxoplasma gondii. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015 |
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