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Campylobacter sp in organs and meconium of day-old broiler chicks derived from naturally infected breeder hens 89
Fonseca,BB; Soncini,RA; Vieira,FL; Siqueira,MS; Guimarães,AR; Beletti,ME; Rossi,DA.
Campylobacter sp is an important agent that causes foodborne infection, particularly in food of poultry origin. Therefore, the efficient control of the transmission routes in chicken farms is of outmost importance to prevent it from spreading. In chicken farms, the main transmission route of this microorganism is horizontal, as the vertical route continues to be the object of inconclusive researches. The objective of this study was to verify the presence of Campylobacter sp in breeder hens, meconium, and other organs of day-old chicks derived from these breeders in order to obtain information on vertical transmission. Microbiological analyses were performed, using cloacal swabs from 279 breeder hens. Positive breeders were segregated, and the presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter sp; Chickens; Vertical transmission; Epidemiology.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400010
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Biological characterization of a myotoxin phosphoplipase A2 homologue purified from the venom of the snake Bothrops moojeni 80
Queiroz,MR; Mamede,CC; Fonseca,KC; Canabrava,LCMN; França,LV; Silva,MC; Stanziola,L; Beletti,ME; Canabrava,HAN; Oliveira,F.
A myotoxin phospholipase A2 homologue, BmooMtx, was isolated from the venom of Bothrops moojeni by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. SDS-PAGE showed the enzyme to be a monomer with a molecular weight of 16,500. BmooMtx induced release of creatine kinase and morphological analyses indicated that it provoked an intense myonecrosis, with visible leukocyte infiltrate and damaged muscle cells 24 hours after injection. Anti-BmooMTx antibodies partially neutralized the myotoxic activity of BmooMtx and crude B. moojeni venom, as judged by determination of plasma creatine kinase levels and histological evaluation of skeletal muscle in mice. Anti-BmooMTx antibodies were effective in reducing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops moojeni; Hyperalgesia; Myotoxin; Edema.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100007
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Proposal of a Standard for the Condemnation for Turkey Carcasses Due to Fowlpox 89
Ferreira,BC; Couto,RM; Ecco,R; Coelho,HE; Rossi,DA; Beletti,ME; Silva,PL.
ABSTRACT This study aimed at proposing a new technical criteria for condemnation of turkey carcasses due to fowlpox in turkeys as a contribution for the work of the Brazilian Federal Meat Inspection Service. Skin samples from 30 carcasses of a flock of 840 turkeys (Meleagris gallopavo), previously vaccinated for fowlpox and slaughtered in June 2013, were collected. Samples were submitted to histological examination under light microscopy. The virus was identified using standard PCR techniques. The main histological findings were hyperplasia and hydropic degeneration of the epithelium and the presence of intracytoplasmic eosinophilic inclusion bodies. PCR results yielded 83.3% positive and 16.7% negative samples. Fowlpox virus is species specific, and there...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Condemnations; Fowlpox; Processing plant; SIF.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200225
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Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens 89
Fonseca,BB; Ferreira Júnior,A; Santos,JP dos; Coelho,LR; Rossi,DA; Melo,RT; Mendonça,EP; Araújo,TG; Alves,RN; Beletti,ME.
Abstract Campylobacteriosis is a worldwide foodborne zoonosis disease caused by Campylobacter jejuni. This microorganism is considered a commensal bacterium in chicken hosts. C. jejuni produces epithelial cell modifications and induces a cytokine gene transcription innate immunity repertoire. In the present study, we describe the invasiveness, morphological cellular modifications, and transcript level expressions of innate immune cytokines from C. jejuni-inoculated chicken ileum explants. C. jejuni was internalized by epithelial ileum cells at 15 minutes postinoculation (p.i.) and was detected intracellularly for 4hs (p.i.). Inoculated explants displayed significant increases in cell height. C. jejuni induced a significant elevation of Transforming Growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; Chicken; Explants; TGF-b3; IL-1b.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100063
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