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Registros recuperados: 10
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Hordes of Phages in the Gut of the Tilapia Sarotherodon melanotheron 5
Bettarel, Yvan; Combe, Marine; Adingra, Antoinette; Ndiaye, Awa; Bouvier, Thierry; Panfili, Jacques; Durand, Jean-dominique.
Preliminary studies conducted on the human gastro-intestinal tract have revealed that enteric viral communities play a preponderant role in microbial homeostatis. However to date, such communities have never been investigated in the fish gut. Herein, we examined the main ecological traits of viruses in the digestive tract of a euryhaline fish, the tilapia Sarotherodon melanotheron. Individuals were collected at 8 different sites in Senegal covering a salinity gradient from 3 to 104 parts per thousand, and showing large disparities in their organic pollutant concentrations. Results showed that the gut of S. melanotheron is home to a highly abundant viral community (0.2-10.7 x 10(9) viruses ml(-1)), distinct from the surrounding water, and essentially...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00452/56387/75058.pdf
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Skin microbiome of coral reef fish is highly variable and driven by host phylogeny and diet 5
Chiarello, Marlene; Auguet, Jean-christophe; Bettarel, Yvan; Bouvier, Corinne; Claverie, Thomas; Graham, Nicholas A. J.; Rieuvilleneuve, Fabien; Sucre, Elliot; Bouvier, Thierry; Villeger, Sebastien.
Background: The surface of marine animals is covered by abundant and diversified microbial communities, which have major roles for the health of their host While such microbiomes have been deeply examined in marine invertebrates such as corals and sponges, the microbiomes living on marine vertebrates have received less attention. Specifically, the diversity of these microbiomes, their variability among species, and their drivers are still mostly unknown, especially among the fish species living on coral reefs that contribute to key ecosystem services while they are increasingly affected by human activities. Here, we investigated these knowledge gaps analyzing the skin microbiome of 138 fish individuals belonging to 44 coral reef fish species living in the...
Tipo: Text Palavras-chave: Tropical; Teleost; Microbiota; Phylogenetic diversity; Phylosymbiosis; Phylogenetic signal.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56565/74934.pdf
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Exceptional but vulnerable microbial diversity in coral reef animal surface microbiomes 5
Chiarello, Marlène; Auguet, Jean-christophe; Graham, Nicholas A. J.; Claverie, Thomas; Sucré, Elliott; Bouvier, Corinne; Rieuvilleneuve, Fabien; Restrepo-ortiz, Claudia Ximena; Bettarel, Yvan; Villéger, Sébastien; Bouvier, Thierry.
Coral reefs host hundreds of thousands of animal species that are increasingly threatened by anthropogenic disturbances. These animals host microbial communities at their surface, playing crucial roles for their fitness. However, the diversity of such microbiomes is mostly described in a few coral species and still poorly defined in other invertebrates and vertebrates. Given the diversity of animal microbiomes, and the diversity of host species inhabiting coral reefs, the contribution of such microbiomes to the total microbial diversity of coral reefs could be important, yet potentially vulnerable to the loss of animal species. Analysis of the surface microbiome from 74 taxa, including teleost fishes, hard and soft corals, crustaceans, echinoderms,...
Tipo: Text Palavras-chave: Skin microbiota; Phylogenetic diversity; Conservation; Marine biodiversity; Octocorallia; Scleratinia.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74121/73596.pdf
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High diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species 5
Chiarello, Marlene; Villeger, Sebastien; Bouvier, Corinne; Bettarel, Yvan; Bouvier, Thierry.
Animal-associated microbiotas form complex communities, which are suspected to play crucial functions for their host fitness. However, the biodiversity of these communities, including their differences between host species and individuals, has been scarcely studied, especially in case of skin-associated communities. In addition, the intraindividual variability (i.e. between body parts) has never been assessed to date. The objective of this study was to characterize skin bacterial communities of two teleostean fish species, namely the European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), using a high-throughput DNA sequencing method. In order to focus on intrinsic factors of host-associated bacterial community variability,...
Tipo: Text Palavras-chave: Sparus aurata; Dicentrarchus labrax; Skin microbiome; Next generation sequencing; Phylogenetic diversity.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00277/38801/37547.pdf
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The mangrove's contribution to people: Interdisciplinary pilot study of the Can Gio Mangrove Biosphere Reserve in Viet Nam 5
Cormier-salem, Marie-christine; Van Trai, Nguyen; Burgos, Ariadna; Durand, Jean-dominique; Bettarel, Yvan; Klein, Judith; Duc Huy, Hoang; Panfili, Jacques.
The main objective of this pilot study, conducted in June 2015 in the Can Gio Mangrove Biosphere Reserve (Can Gio MBR, Viet Nam), was to develop an interdisciplinary approach to assess some key services provided by reforested mangroves subject to external pressures and varying management policies. We focused on the abundance of viruses, bacteria, endo-and epi-and macrofauna and the diversity of crabs in the mangrove and the exploitation of its resources. The main social finding was that the local inhabitants are aware of the levels of protection of the different zones within the Can Gio MBR and respect them. The core and the buffer zones seem to present a similar ecological status. Genotyping showed a low level of crab diversity although there were many...
Tipo: Text Palavras-chave: Mangrove; Ecosystem services; Biodiversity; Local knowledge; Coastal environment; Interdisciplinary framework.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00417/52852/74762.pdf
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Therapeutic Potential of a New Jumbo Phage That Infects Vibrio coralliilyticus, a Widespread Coral Pathogen 5
Jacquemot, Loic; Bettarel, Yvan; Monjol, Joanne; Corre, Erwan; Halary, Sebastien; Desnues, Christelle; Bouvier, Thierry; Ferrier-pages, Christine; Baudoux, Anne-claire.
Biological control using bacteriophages is a promising approach for mitigating the devastating effects of coral diseases. Several phages that infect Vibrio coralliilyticus, a widespread coral pathogen, have been isolated, suggesting that this bacterium is permissive to viral infection and is, therefore, a suitable candidate for treatment by phage therapy. In this study, we combined functional and genomic approaches to evaluate the therapeutic potential of BONAISHI, a novel V. coralliilyticus phage, which was isolated from the coral reef in Van Phong Bay (Vietnam). BONAISHI appears to be strictly lytic for several pathogenic strains of V. coralliilyticus and remains infectious over a broad range of environmental conditions. This candidate has an unusually...
Tipo: Text Palavras-chave: Phage therapy; Coral disease; Vibrio coralliilyticus; Viral genomics; Phage-host interactions.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00465/57717/74946.pdf
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Enumerating Viruses in Coral Mucus 5
Leruste, Amandine; Bouvier, Thierry; Bettarel, Yvan.
The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate.
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00092/20371/18048.pdf
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Procarcinogens - Determination and Evaluation by Yeast-Based Biosensor Transformed with Plasmids Incorporating RAD54 Reporter Construct and Cytochrome P450 Genes 5
Ngoc Bui, Van; Thu Huyen Nguyen, Thi; Thanh Mai, Chi; Bettarel, Yvan; Yen Hoang, Thi; Thuy Linh Trinh, Thi; Hai Truong, Nam; Ha Chu, Hoang; Thanh Thanh Nguyen, Vu; Duc Nguyen, Huu; Woelfl, Stefan.
In Vietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. In the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. One plasmid is carrying human CPR and CYP (CYP3A4, CYP2B6, or CYP2D6) genes, while the other contains the RAD54-GFP reporter construct. The three resulting coexpression systems bearing both CPR-CYP and RAD54-GFP expression cassettes were designated as CYP3A4/CYP2B6/CYP2D6 + RAD54 systems, respectively and used to detect and evaluate the genotoxic potential of...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00625/73740/74114.pdf
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Viral and Bacterial Epibionts in Thermally-Stressed Corals 5
Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan.
The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of...
Tipo: Text Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf
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Coral-associated viruses and bacteria in the Ha Long Bay, Vietnam 5
Thu Pham, The; Thuoc Chu, Van; Viet Ha Bui, Thi; Thuy Nguyen, Thanh; Huy Tran, Quang; Ngoc Mai Cung, Thi; Bouvier, Corinne; Brune, Justine; Villeger, Sebastien; Bouvier, Thierry; Bettarel, Yvan.
Viruses inhabiting the surface mucus layer of scleractinian corals have received little ecological attention so far. Yet they have recently been shown to be highly abundant and could even play a pivotal role in coral health. A fundamental aspect that remains unresolved is whether their abundance and diversity change with the trophic state of their environment. The present study examined the variability in the abundance of viral and bacterial epibionts on 13 coral species collected from 2 different sites in the Ha Long Bay, Vietnam: one station heavily affected by anthropogenic activity (Cat Ba Island) and one protected offshore station (Long Chau Island). In general, viral abundance was significantly higher in coral mucus (mean = 10.6 +/- 2.0 x 10(7)...
Tipo: Text Palavras-chave: Viruses; Coral-associated bacteria; Mucus; Symbionts; Coral reefs.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00627/73870/73680.pdf
Registros recuperados: 10
Primeira ... 1 ... Última
 

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