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DUBIELA, C. R.; FAJARDO, T. V. M.; SOUTO, E. R.; NICKEL, O.; EIRAS, M.; REVERS, L. F.. |
The aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus... |
Tipo: Artigo de periódico |
Palavras-chave: Viticultura; Uva; Doença de planta; Vírus; Diagnostico.; Betaflexiviridae; Closteroviridae.. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/960405 |
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