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| Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.. |
| This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses. |
| Tipo: Text |
Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf |
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| Boukouvala, Evridiki; Cariani, Alessia; Maes, Gregory E.; Sevilla, Rafael G.; Verrez-bagnis, Veronique; Jerome, Marc; Guarniero, Ilaria; Monios, Georgios; Tinti, Fausto; Volckaert, Filip A.m.; Bautista, José M.; Krey, Grigorios. |
| Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. Here we describe a simple restriction fragment length-based diagnostic test, which differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, we show that the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property which can also be used to distinguish the... |
| Tipo: Text |
Palavras-chave: Solea solea; Solea aegyptiaca; Species diagnosis; Cytochrome b gene; EcoR V restriction fragment length; White muscle fatty acid composition. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19974/17641.pdf |
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