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Molecular cloning, gene structure and expression profile of two mouse peroxisomal 3-ketoacyl-CoA thiolase genes Inra
Chevillard, G.; Clémencet, M.C.; Etienne, P.; Martin, P.; Pineau, T.; Latruffe, N.; Nicolas-Francès, V..
Tipo: Journal Article Palavras-chave: EXPRESSION DES GENES; ACTIVITE ENZYMATIQUE; ADN COMPLEMENTAIRE; FOIE; EXPERIMENTATION ANIMALE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0500023809110490&uri=/notices/prodinra1/2009/10/
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Effects of oxysterols on cell viability, inflammatory cytokines, VEGF, and reactive oxygen species production on human retinal cells: cytoprotective effects and prevention of VEGF secretion by resveratrol Inra
Dugas, B.; Charbonnier, S.; Baarine, M.; Ragot, K.; Delmas, D.; Menetrier, F.; Lherminier, J.; Malvitte, L.; Khalfaoui, T.; Bron, A.; Creuzot Garcher, C.; Latruffe, N.; Lizard, G..
Background and aimsOxysterols are assumed to play important roles in age-related macular degeneration, a major cause of blindness. So we characterized the cytotoxic, oxidative, inflammatory, and angiogenic activities of oxysterols (7β-hydroxycholesterol (7β-OH), 7-ketocholesterol (7KC), 25-hydroxycholesterol (25-OH)) in human retinal ARPE-19 cells, and evaluated the protective effects of resveratrol (Rsv: 1 μM), a polyphenol from red wine.MethodsARPE-19 cells were treated with 7β-OH, 7KC, or 25-OH (5–40 μg/mL; 24–48 h) without or with Rsv. Cell viability was determined using trypan blue and the MTT assay. Cell death was characterized by electron microscopy and in situ detection of activated caspases with fluorochrome-labeled inhibitors of caspases....
Tipo: Journal Article Palavras-chave: CELLULES RÉTINIENNES; DEGENERESCENCE MACULAIRE; DMLA ARPE-19 CELLS; CASPASE-INDEPENDENT CELL DEATH; INFLAMMATORY CYTOKINES; OXYSTEROLS; PHOSPHOLIPIDOSIS; RESVERATROL; REACTIVE OXYGEN SPECIES; VEGF.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201135a1139e&uri=/notices/prodinra1/2011/06/
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