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Biochemical and pharmacological study of venom of the wolf spider Lycosa singoriensis 80
Liu,ZH; Qian,W; Li,J; Zhang,Y; Liang,S.
The wolf spider Lycosa singoriensis is a large and venomous spider distributed throughout northwestern China. Like other spider venoms, the wolf spider venom is a chemical cocktail. Its protein content is 0.659 mg protein/mg crude venom as determined by the Lowry method. MALDI-TOF analysis revealed that the venom peptides are highly diverse and may be divided into three groups characterized by three independent molecular ranges: 2,000 to 2,500 Da, 4,800 to 5,500 Da and 7,000 to 8,000 Da, respectively. This molecular distribution differs substantially from those of most spider venoms studied so far. This wolf spider venom has low neurotoxic action on mice, but it can induce hemolysis of human erythrocytes. Furthermore, the venom shows antimicrobial activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spider; Lycosa singoriensis; Crude venom; MALDI-TOF; Antimicrobial activity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100008
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Effect of Feeding Conditions on the Methylation Status of Fatp1 Gene in Chicken Breast Muscle 89
Gao,G; Wang,H; Zhao,X; Li,Q; Wang,C; Li,J; Zhang,K; Zhong,H; Wang,Q.
ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feeding conditions; Chicken; FATP1; Meat quality; Intramuscular fat.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055
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Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds 89
Zhang,B; Xiang,D; Yang,R; Yang,L; Li,J; Zhao,Z.
ABSTRACT Adipose differentiation-related protein (ADFP) is a fatty acid-binding protein that can promote the absorption of long-chain fatty acids. However, few results have been published regarding its role in Yunnan Native chicken breeds. The aim of this study was to determine ADFP gene tissue-specific expression in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) by RT-qPCR. The ontogenetic expression levels of the ADFP gene were significantly different during growth and development phases in the subcutaneous fat, liver, and pectoralis muscle of PC, and in the subcutaneous fat, liver, and pectoralis muscle of WBC (p<0.05). Individual tissue-differential expression levelswere detectedon d 91 and 112 in PC, with highest levels determined in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ADFP; Carcass fatness; Piao chickens; RT-qPCR; Wuliangshan black-bone chickens.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318
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Characterization and Differentiation of Chicken Mycoplasma Isolates Using 16S-23S Intergenic Spacer Region Sequencing 89
Santos,FF; Li,J; Brandão,MDM; Machado,LS; Soares,NM; Nascimento,ER; Khan,MI; Pereira,VLA.
ABSTRACT The objective of this study was to identify the species and characterize the genetic relationships among mycoplasma isolates from commercial layer hen flocks using 16S-23S rDNA intergenic spacer region (IGSR) sequencing. Twenty-one isolates were obtained from samples collected from commercial layer flocks in four Brazilian states: São Paulo, Minas Gerais, Rio de Janeiro and Espírito Santo. The isolates were recovered from the São Paulo, Rio de Janeiro and Espírito Santo states. Eleven isolates were originated from tracheal swabs, five from shell gland swabs and five from ovary fragment collection. The 16S-23S rDNA IGSR of isolates were amplified by PCR, and the obtained products were subsequently sequenced. The consensus of each isolate was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma gallinaceum; Mycoplasma pullorum; Mycoplasma gallisepticum; Intergenic spacer region; 16S-23S.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200309
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