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| ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z.. |
| Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after... |
| Tipo: Artigo de periódico |
Palavras-chave: Billygoats; CTC; Caprino; Ovino; Bovino; Bode; Carneiro; Reprodução Animal; Sêmen; Heparina; Espermatozóide; Sheep; Goats; Cattle; Rams; Bulls; Animal reproduction; Sperm capacitation; Assisted reproductive technologies; Heparin. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119218 |
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| OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; SOUZA FABJAN, J. M. G.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z.. |
| Abstract: The success of IVF and subsequent development of embryos are directly related to sperm selection and quality. This study aimed to evaluate the effect of different forces and time of centrifugation in mini-Percoll (MP) techniques for sperm selection on capacitation status and plasma membrane (PM) integrity of ram frozen-thawed sperm. Commercial semen from 10 rams of Santa Inês breed, aging 2-5 years old, were used. At post-thawing (PT), the PM integrity was evaluated by CASA using the SCA® system (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Spain). For traditional Percoll (Sigma Chemical, St. Louis, USA), a 2 mL-gradient (90/45% density) was subjected to a 700 x g centrifugation for 10 min followed by 200 x g for 5... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Sperm selection; Sperm preparation; Mini-Percoll; Ovino; Ovelha; Reprodução animal; Espermatozóide; Sêmen; Sheep; Spermatozoa. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1052416 |
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| OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z.. |
| This study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest... |
| Tipo: Separatas |
Palavras-chave: Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection.; Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen.; Goats; Reproduction; Spermatozoa.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035586 |
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| OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z.. |
| Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for... |
| Tipo: Artigo de periódico |
Palavras-chave: Sperm preparation; CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques.; Ovino; Espermatozóide; Sêmen; Reprodução animal; Sheep; Reproduction; Spermatozoa; Treaties; Sperm capacitation.. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1070178 |
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| OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z.. |
| Abstract: Sperm capacitation is an essential event for fertilization; however, it decreases the sperm lifespan and viability. The aim of this study was to evaluate the effects of four sperm selection techniques on sperm capacitation and viability after incubation. A pool of frozen-thawed sperm from 10 Santa Inês rams was used. The samples were submitted to one of the following sperm selection techniques: sperm washing, Percoll gradient, mini-Percoll gradient, Swim-up and control group. At mini-Percoll technique, was used 400 microliters of 90% and 45% gradients and a centrifugation at 500 xg for 5 minutes. In Percoll, was used 1 mL of each gradient and a centrifugation at 700 xg for 10 minutos. During Swim-up, the sperm was incubated in 1 ml of SPERM-TALP... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Longevidade espermática; Seleção espermática; Raça Santa Inês; Sperm longevity; Sperm selection; Embryo Technology.; Ovino; Carneiro; Reprodução animal; Transferência de Embrião; Sêmen; Espermatozóide; Sheep; Reproduction; Rams.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074502 |
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