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Avaliação clínica da infecção experimental de bezerros com Salmonella Dublin Arq. Bras. Med. Vet. Zootec.
Silva,D.G.; Silva,P.R.L.; Fagliari,J.J.; Ávila,F.A; Alessi,A.C.; Oliveira,R.G..
The clinical conditions of healthy calves infected with experimental 10(8)CFU of Salmonella Dublin were evaluated and the viability of the experimental model in disease induction in calves was verified. Twelve 10 to 15-day-old male Holstein calves were examined. They were allocated into two groups, control and experimentally infected with 10(8)CFU of Salmonella Dublin. Animals were submitted to clinical examination after inoculation and at every 12 hours, during seven days after the experimental infection. Samples of rectal swabs were collected for Salmonella Dublin isolation. All animals had severe diarrhea, with mucus and bleeding, 12 to 84 hours after the experimental infection with Salmonella Dublin, accompanied by fever, dehydration and respiratory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bezerro; Salmonella Dublin; Salmonelose.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100034
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Proteinograma sérico de veados-catingueiro (Mazama gouazoubira) criados em cativeiro obtido por eletroforese em gel de agarose e de poliacrilamida (SDS PAGE) Arq. Bras. Med. Vet. Zootec.
Santana,A.M.; Fagliari,J.J.; Camargo,C.M.S.; Santana,A.E.; Duarte,J.M.B.; Silva,P.R.L..
The serum protein concentrations of brown brocket deer (Mazama gouazoubira) obtained by agarosis gel and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel were determined from blood samples of ten adult healthy animals (six females and four males), monthly collected in the morning, during 12 months. The animals, maintained in individual stable and protected from noise, received ad libitum a diet of comercial ration and green roughage. Serum protein concentrations in agarosis gel revealed the presence of four protein fractions: albumin, alphaglobulin, betaglobulin, and gammaglobulin. Only serum concentrations of albumin were influenced by season, being values in spring higher than values in summer (4.15 x 3.64g/dl). Serum concentrations of albumin (4.05...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Veado-catingueiro; Proteinograma; Eletroforese; SDS-PAGE.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600039
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Avaliação da reação em cadeia da polimerase e do isolamento bacteriológico convencional na detecção de Salmonella Dublin em amostras de fezes de bezerros infectados experimentalmente Arq. Bras. Med. Vet. Zootec.
Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J..
The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035
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Efficacy of florfenicol and intravenous fluid therapy for treatment of experimental salmonellosis in newborn calves Arq. Bras. Med. Vet. Zootec.
Silva,D.G.; Silva,P.R.L.; Fagliari,J.J..
The efficacy of florfenicol associated or not to intravenous fluid therapy for treatment of Salmonella Dublin-infected calves was determined. Twenty-four healthy 10 to 15-day-old Holstein calves were randomly allotted into four groups, with six animals each: control (group 1); infected with 10(8)CFU Salmonella Dublin and not treated (group 2); infected with 10(8)CFU Salmonella Dublin and treated with florfenicol (group 3); and infected with 10(8)CFU Salmonella Dublin and treated with florfenicol associated to fluid therapy (group 4). All animals were submitted to physical examination just before inoculation and every 24 hours, during seven days after experimental infection. Rectal swabs and blood samples were collected for Salmonella Dublin isolation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calf; Salmonella Dublin; Florfenicol; Lactated Ringer's solution.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300001
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Hemograma e perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin Arq. Bras. Med. Vet. Zootec.
Silva,D.G.; Silva,P.R.L.; Fagliari,J.J..
O objetivo do estudo foi avaliar o hemograma e o perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin. Foram utilizados 12 bezerros sadios da raça Holandesa com 10 a 15 dias de idade, distribuídos aleatoriamente em dois grupos experimentais: grupo-controle (n= 6) e grupo infectado com 10(8)UFC de Salmonella Dublin (n=6). Os bezerros foram submetidos ao exame físico diário, e as amostras de sangue foram coletadas minutos antes da inoculação (0h) e 24, 48, 72, 96, 120, 144 e 168h após a inoculação. Além do hemograma e das análises hemogasométricas, foram mensuradas as atividades séricas das enzimas aspartato aminotransferase (AST), fosfatase alcalina (ALP), creatina cinase (CK),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bezerro; Salmonella Dublin; Diarreia; Hemograma; Eletrólitos.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200002
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