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Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle BJMBR
Leite,L.N.; Gonzaga,N.A.; Tirapelli,D.P.C.; Tirapelli,L.F.; Tirapelli,C.R..
The aim of the present study was to determine the mechanisms underlying the relaxant effect of adrenomedullin (AM) in rat cavernosal smooth muscle (CSM) and the expression of AM system components in this tissue. Functional assays using standard muscle bath procedures were performed in CSM isolated from male Wistar rats. Protein and mRNA levels of pre-pro-AM, calcitonin receptor-like receptor (CRLR), and Subtypes 1, 2 and 3 of the receptor activity-modifying protein (RAMP) family were assessed by Western immunoblotting and quantitative real-time polymerase chain reaction, respectively. Nitrate and 6-keto-prostaglandin F1α (6-keto-PGF1α; a stable product of prostacyclin) levels were determined using commercially available kits. Protein and mRNA of AM, CRLR,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rat cavernosal smooth muscle; Adrenomedullin; Relaxation; Nitric oxide; Calcitonin receptor-like receptor.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000876
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Expression profile of endothelin receptors (ETA and ETB) and microRNAs-155 and -199 in the corpus cavernosum of rats submitted to chronic alcoholism and diabetes mellitus BJMBR
Gonçalves,F.Z.; Lizarte Neto,F.S.; Novais,P.C.; Gattas,D.; Lourenço,L.G.; de Carvalho,C.A.M.; Tirapelli,D.P.C.; Molina,C.A.F.; Tirapelli,L.F.; Tucci Jr,S..
Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alcoholism; Corpus cavernosum; MiRNAs; Endothelin receptors.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300615
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Kaurene diterpene induces apoptosis in U87 human malignant glioblastoma cells by suppression of anti-apoptotic signals and activation of cysteine proteases BJMBR
Lizarte Neto,F.S.; Tirapelli,D.P.C.; Ambrosio,S.R.; Tirapelli,C.R.; Oliveira,F.M.; Novais,P.C.; Peria,F.M.; Oliveira,H.F.; Carlotti Junior,C.G.; Tirapelli,L.F..
Gliomas are the most common and malignant primary brain tumors in humans. Studies have shown that classes of kaurene diterpene have anti-tumor activity related to their ability to induce apoptosis. We investigated the response of the human glioblastoma cell line U87 to treatment with ent-kaur-16-en-19-oic acid (kaurenoic acid, KA). We analyzed cell survival and the induction of apoptosis using flow cytometry and annexin V staining. Additionally, the expression of anti-apoptotic (c-FLIP and miR-21) and apoptotic (Fas, caspase-3 and caspase-8) genes was analyzed by relative quantification (real-time PCR) of mRNA levels in U87 cells that were either untreated or treated with KA (30, 50, or 70 µM) for 24, 48, and 72 h. U87 cells treated with KA demonstrated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kaurenoic acid; Glioblastoma; MiR-21; C-FLIP; Apoptosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100071
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