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Implementation of a Molecular System for Traceability of Beef Based on Microsatellite Markers Chilean J. Agric. Res.
Felmer D,Ricardo; Sagredo D,Boris; Chávez R,Renato; Iraira H,Sergio; Folch M,Carolina; Parra G,Lorena; Catrileo S,Adrián; Ortiz L,Manuel.
Animal products traceability has acquired considerable importance as a security measure in EEC member countries since the food crisis of the mid-nineties. This has led to reinforcing the capacity to manage cattle product quality, with traceability emerging as the main tool to prevent risks to product security and quality as demanded by consumers in developed countries. The practical application of a traceability system for beef, based on molecular markers requires the election of a panel of microsatellites, as well as the optimization of methods of sampling and DNA analysis. In this work, a traceability system for beef based on a panel of 10 microsatellites markers was implemented. Different biological samples were evaluated, such as hair, blood, tissue...
Tipo: Journal article Palavras-chave: Traceability; Microsatellites; Individual identification; Beef.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400004
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Organic Matter Reduces Copper Toxicity for the Earthworm Eisenia fetida in Soils from Mining Areas in Central Chile Chilean J. Agric. Res.
Ávila,Gonzalo; Gaete,Hernán; Sauvé,Sébastien; Neaman,Alexander.
The Aconcagua River basin (Central Chile) is one of the most important agricultural areas in the country. However, several copper (Cu) mining operations are located in the basin. The objective of the study was to determine Cu toxicity for the earthworm Eisenia fetida (Savigny 1826) in the agricultural soils of the basin. We determined the production of cocoons and juveniles of earthworms in the studied soils. The soils differed in the concentrations of organic matter (OM, range 2-6%), pH (range 7.3-8.3), texture (from loamy sand to clay loam), and total Cu concentrations (range 230-960 mg kg-1). Concentrations of Cu and OM in the soils were the variables that determined the earthworms’ biological response. In contrast, pH and texture did not affect this...
Tipo: Journal article Palavras-chave: Aconcagua River; Ecological risk assessment; Cu mining; Trace elements.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200015
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Influence of Water Temperature and Heat Stress on Drinking Water Intake in Dairy Cows Chilean J. Agric. Res.
González Pereyra,Ana Valeria; Maldonado May,Verónica; Catracchia,Carlos Guillermo; Herrero,María Alejandra; Flores,Myriam Celina; Mazzini,Mariana.
Grazing animals are exposed to heat stress and there should be conditions to facilitate water intake. Three experiments were carried out to determine the relationship between water intake, water temperature, and heat stress for Holstein pastured dairy cows. Experiment 1: Drinking behavior of 13 cows was evaluated (for 3 consecutive days) and four time periods were established according to the temperature humidity index (THI): (H1:05:00 to 10:59 h, H2:11:00 to 16:59 h, H3:17:00 to 22:59 h, and H4:23:00 to 04:59 h). The highest mean percentage of drinking bouts (53.2%) was registered in H2 (THI: 74.91 to 83.95). Drinking bout means among time periods were different (p < 0.05), thus showing that heat stress conditions influence drinking behavior....
Tipo: Journal article Palavras-chave: Drinking behavior; Summer time; THI; Grazing dairy farms.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200017
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Millán-INIA, New Variety of High Quality Early Spring Bread Wheat for Irrigated Soils in Central-Southern Chile Chilean J. Agric. Res.
Matus,Iván; Madariaga,Ricardo; Jobet,Claudio; Zúñiga,Javier; Alfaro,Christian.
Millán-INIA is a variety of spring bread wheat (Triticum aestivum L.) originating from a cross carried out in the Wheat Plant Breeding Project of the Instituto de Investigaciones Agropecuarias (INIA), in the Centro Regional de Investigación Quilamapu in 1995. This is a spring wheat variety with an early to medium-early head emergence and upright growth habit in the seedling stage. The adult plant is low to medium height and varies between 75 and 90 cm, with a mean of 85 cm. The spike is white with long awns along its full length. The grain is large-sized, white, and vitreous. The weight of 1000 grains varies between 50 and 59 g. It was sown in mid-August at the Santa Rosa Experimental Station (36°31’ S; 71°54’ W), Chillán. Head emergence occurred 88 to 89...
Tipo: Journal article Palavras-chave: Spring wheat; New variety; Millán-INIA.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000200019
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Evaluation of On-Farm Quick Tests on Slurries from Southern Chile Dairy Farms Chilean J. Agric. Res.
Salazar S,Francisco; Rosas U,Marianela.
Currently in Chile, there is a lack of tools to determine the real-time nutrient contribution of slurries. This study aims to evaluate three quick tests for their accuracy in estimating the total contents of N (TN), P (TP), K (TK), and ammonium-N (N-NH4+) in dairy slurries. The quick tests included the Agros Nova Meter, Quantofix N Volumeter, and a hydrometer. While the Agros and Quantofix meters directly measure the ammonium-N content; the hydrometer indirectly estimates the total content of N, P, and K based on linear relationships with the slurry DM. Samples (73) were collected from dairy farms along Southern Chile (39° to 44° S and 71 to 73° W). Analyses were carried out on laboratory conditions using conventional techniques as well as using each of...
Tipo: Journal article Palavras-chave: Slurry; Organic residue; Chemical analysis; Nutrients.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300021
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Analysis of Agromorphological Descriptors to Differentiate between Duke Cherry (Prunus x gondouinii (Poit. &amp; Turpin)Rehd.) and Its Progenitors: Sweet Cherry (Prunus avium L.) and Sour Cherry (Prunus cerasus L.) Chilean J. Agric. Res.
Pérez,Rodrigo; Navarro,Florentino; Sánchez,María Ángeles; Ortíz,Jesús María; Morales,Remedios.
The rapid identification of the hybrids between sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) is not easy. In order to resolve this problem, 18 Spanish sweet, sour and duke cherry cultivars were surveyed and characterized using 43 agromorphological descriptors evaluated in flowers, leaves, dormant 1-yr-old shoots, fruits, and trees during 2005 and 2006. Based on quantitative parameters, ANOVA and stepwise discriminant analysis (SDA) were carried out. For qualitative descriptors, statistical comparisons were done by means of the chi-square (&#967;2) test. As result of the study, two quantitative (titratable acidity and number of lenticels) and six qualitative descriptors (shape of the central and lateral lobes in the internal bracts...
Tipo: Journal article Palavras-chave: Rosaceae; Characterization; Quantitative parameters; Qualitative parameters; SDA analysis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000100004
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Bio-Economic and Qualitative Impact of Reduced Herbicide use in Direct Seeded Fine Rice Through Multipurpose Tree Water Extracts Chilean J. Agric. Res.
Khaliq,Abdul; Matloob,Amar; Riaz,Yasir.
Weed control program should be environmentally benign and cost-effective so that reduced herbicide use can help meet these goals. Field trials were conducted to assess the bio-economic and qualitative impact of reduced doses (25 and 50% of label dose) of a postemergence pyrimidinyloxybenzoic acid herbicide (bispyribac-sodium) applied alone or in combination with multipurpose tree (eucalyptus Eucalyptus camaldulensis Dehnh., mango Mangifera indica L., and mulberry Morus alba L.) water extracts in direct seeded rice (Oryza sativa L.) fields. The label dose of bispyribac-sodium and penoxsulam along with weed control were included for comparison. Tank mixing of multipurpose tree water extracts with reduced herbicide doses accounted for > 55% suppression in...
Tipo: Journal article Palavras-chave: Reduced dose; Weed suppression; Yield; Kernel quality; Oryza sativa.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300008
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Performance of Late Sown Wheat in Response to Foliar Application of Moringa oleifera Lam. Leaf Extract Chilean J. Agric. Res.
Yasmeen,Azra; Basra,Shahzad Maqsood Ahmed; Ahmad,Rashid; Wahid,Abdul.
A rise in temperature during early spring inducing early maturity is a key yield-reducing factor in late sown wheat (Triticum aestivum L.). Moringa oleifera Lam. leaves are rich in zeatin, a cytokinin that plays a role in delaying leaf senescence, in addition to other growth-enhancing compounds such as ascorbates, phenolics, and minerals. The objective of this study was to optimize dose and optimum growth stage for foliar-applied moringa leaf extract (MLE) and its role in delaying leaf senescence in late sown wheat. The wheat crop was shown on 16 December 2008; MLE (diluted 30 times) was applied at different growth stages from tillering to heading and heading alone and distilled water was sprayed as a control. All the MLE treatment results were better than...
Tipo: Journal article Palavras-chave: Crop maturity; Seasonal leaf area duration; Late sowing; Wheat.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100015
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Effect of Harvest Time and L-Cysteine as an Antioxidant on Flesh Browning of Fresh-Cut Cherimoya (Annona cherimola Mill.) Chilean J. Agric. Res.
Campos-Vargas,Reinaldo; Defilippi,Bruno G; Romero Q,Paula; Valdés G,Héctor; Robledo M,Paula; Prieto E,Humberto.
Browning development is the most important factor limiting the quality of fresh-cut cherimoya (Annona cherimola Mill.). However, there is little information available about its causes and methods of control. The effectiveness of L-cysteine (0.125, 0.25 and 0.5%) in fresh-cut cherimoya harvested on two occasions (October and November) and stored for 6 and 12 days at 0 °C was studied. In order to understand the biological basis of browning, polyphenol oxidase (PPO) enzyme activity and total phenolic content in fresh-cut pieces were measured. Quality measurements and sensory analysis indicated that 0.5% L-cysteine was somewhat effective in reducing browning development, without affecting other quality attributes. In terms of physiological parameters, PPO...
Tipo: Journal article Palavras-chave: Antioxidants; Polyphenol oxidase; Phenolics; Sensorial evaluation.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000300001
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Nymphicidal and Ovipositional Efficacy of Seaweed Sargassum tenerrimum (J. Agardh) against Dysdercus cingulatus (Fab.) (Pyrrhocoridae) Chilean J. Agric. Res.
Sahayaraj,Kitherian; Jeeva,Y. Mary.
We investigated the role of a brown seaweed alga, Sargassum tenerrimum (J. Agardh), against Dysdercus cingulatus (Fab.), which is an economically important cotton pest in many parts of Asia. Sargassum tenerrimum has been used as a source of fertilizers and drugs, but there is no information available in the literature about its use in ecofriendly pest management. The impact of benzene (BN), chloroform (CH), and a mixture of benzene and chloroform (BNCH) extracts, as well as two chromatographic fractions of BNCH (FR1 and FR2) were investigated for their insecticidal and ovipositional properties against D. cingulatus. The BE extract showed the best insecticidal activity (LC50 = 0.009%), higher than BNCH (LC50 = 0.021%) and CH extracts (LC50 = 0.2481%), and...
Tipo: Journal article Palavras-chave: Cotton pest; Nymphicidal; Ovipositional; Seaweed; Pest management.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100024
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Effect of feeding Acacia saligna (Labill.) H.L. Wendl. on goats stabled during late pregnancy and lactation Chilean J. Agric. Res.
Meneses R,Raul; Olivares V,Yohana; Martinoli S,Milenko; Flores P,Hugo.
Acacia saligna (Labill.) H.L. Wendl. forage is an alternative feed supply for goats during dry periods It was used as feed during pregnancy and lactation to evaluate production response and some blood parameters. Six animals in each group were fed with 0, 25, 50, 75, and 100% of acacia as alfalfa (Medicago sativa L.) hay replacement in a completely randomized design. Forage chemical analysis was done to calculate nutrient intake. Blood samples were analyzed for albumin, urea N, globulin, total protein, Ca, and P. Productive parameters were analyzed by ANOVA, Duncan, and regression analyses between acacia and dry matter (DM), crude protein (CP), metabolizable energy (ME), and milk production. Acacia consumption during pregnancy was 65.5% of control,...
Tipo: Journal article Palavras-chave: Acacia saligna; Pregnancy; Lactation; Milk production; Goats; Albumins.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400014
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Phosphorus absorption and use efficiency by Lotus spp. under water stress conditions in two soils: A pot experiment Chilean J. Agric. Res.
Castillo,Carolina; Acuña,Hernán; Zagal,Erick; Inostroza,Luis.
The response to P and water deficiencies of forage Lotus species has not been sufficiently studied in the Andisol and Vertisol soil orders in Chile's marginal areas. A pot experiment under cover was carried out between October 2007 and March 2008 to study the effects of P and soil water availability (SWA) on DM production, P absorption, and P use efficiency in Lotus spp. The experiment included three Lotus (L. corniculatus L., L. tenuis Waldst. &amp; Kit. ex Willd., and L. uliginosus Schkuhr) species, two soils (Andisol and Vertisol), two contrasting P levels (low and high), and two SWA levels (10% and 100%). A completely randomized design with a 3 x 2 x 2 x 2 factorial arrangement with four replicates was used. Accumulated shoot and root DM, P...
Tipo: Journal article Palavras-chave: Lotus spp.; Volcanic soils; Clay soils; Mycorrhizal colonization.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100005
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No direct effects of resistant soybean cultiva IAC-24 on Podisus nigrispinus (Heteroptera: Pentatomidae) Chilean J. Agric. Res.
Cola Zanuncio,José; Azevedo de Freitas,Fernando; de Souza Tavares,Wagner; Lourenção,André Luiz; Vinha Zanuncio,Teresinha; Serrão,José Eduardo.
The parameters of survival, development and reproduction of Podisus nigrispinus Dallas, 1851 (Heteroptera: Pentatomidae) may be affected by feeding on soybean (Glycine max L. Merr.; Fabaceae) cultivars. The direct effect was evaluated of the insect-resistant soybean 'IAC-24' (insect-susceptible soybean 'UFVS-2006' + pupae of Tenebrio molitor L., 1758 Coleoptera: Tenebrionidae versus 'IAC-24' plants + pupae of T. molitor) in the nymph and adult stages of the predator P. nigrispinus. The duration of the nymph stage was similar between treatments, but 'IAC-24' reduced the duration of the IV instar of this predator. The survival of P. nigrispinus was similar between treatments in the instars I, II, IV and V and in the nymph stage, but the survival of the III...
Tipo: Journal article Palavras-chave: Asopinae; Biological control; Integrated pest management; Predator; Soybean.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000400011
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Isolation and Selection of Epiphytic Yeast for Biocontrol of Botrytis cinerea Pers. on Table Grapes Chilean J. Agric. Res.
Vargas,Marisol; Garrido,Felipe; Zapata,Nelson; Tapia,Maritza.
Botrytis cinerea Pers., the causal agent of gray mold, infects more than 200 plant species. This pathogen has traditionally been controlled by fungicides. However, with the increasing demand for pesticide-free foods new control strategies are needed. The objective of this study was to isolate and select grapevine (Vitis vinifera L.) epiphytic yeasts for the biocontrol of B. cinerea in table grapes. Of the total isolated yeasts (n = 256), 32 exhibited mycelial growth inhibition in dual cultures with a halo > 4 mm, and eight of these isolates inhibited > 90% of conidial germination. When evaluating increasing concentrations on conidial germination inhibition, a dose-dependent response was observed with EC90 values from 0.45 x 10(5) to 0.22 x 10(8)...
Tipo: Journal article Palavras-chave: Gray mold; Antagonistic yeast; Biocontrol agents; Vitis vinifera.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300005
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Relationship between Root Growth of &#39;Thompson Seedless&#39; Grapevines and Soil Temperature Chilean J. Agric. Res.
Callejas R,Rodrigo; Canales P,Pablo; García de Cortázar G. de C,Víctor.
With the purpose of characterizing the growth of the root system of table grapes (Vitis vinifera L.) cv. Thompson Seedless and its relationship with soil temperature, research was carried out during the 2004-2005 growing season in vineyards in the Copiapó and Huasco Valleys. Four plants were chosen and rhizotrons were used to measure the growth of the root system. Measurements were performed once a week from September 2004 to September 2005, estimating the intensity of root growth and its annual distribution in the ranges of 0 to 40, 40 to 80 and 80 to 120 cm of soil depth. Temperature sensors were installed in four plants at 25, 50, 75 and 100 cm of soil depth, and the temperature was registered every 1 h. The thermal diffusivity of the soil was...
Tipo: Journal article Palavras-chave: Roots; Thermal diffusivity; Tablegrape.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400003
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Effect of Pheromone Trap Density on Mass Trapping of Male Potato Tuber Moth Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), and Level of Damage on Potato Tubers Chilean J. Agric. Res.
Larraín S,Patricia; Guillon,Michel; Kalazich,Julio; Graña,Fernando; Vásquez,Claudia.
Potato tuber moth (PTM), Phthorimaea operculella (Zeller), is one of the pests that cause the most damage to potatoes (Solanum tuberosum L.) in both field crops and storage, especially in regions where summers are hot and dry. Larvae develop in the foliage and tubers of potatoes and cause direct losses of edible product. The use of synthetic pheromones that interfere with insect mating for pest control has been widely demonstrated in numerous Lepidoptera and other insect species. An experiment was carried out during the 2004-2005 season in Valle del Elqui, Coquimbo Region, Chile, to evaluate the effectiveness of different pheromone trap densities to capture P. operculella males for future development of a mass trapping technique, and a subsequent decrease...
Tipo: Journal article Palavras-chave: Potato tuber moth; Mass trapping; Pheromone.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200018
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Field Testing of Tomato Chilling Tolerance under Varying Light and Temperature Conditions Chilean J. Agric. Res.
Elizondo,Rafael; Oyanedel,Eduardo.
Chilling tolerance is a desirable trait in commercial tomato varieties, in order to extend the growing season and geographic range. Two Nearly Isogenic Lines (NILs) with introgressions in chromosome 2 and 3 of Solanum habrochaites S. Knapp &amp; D.M. Spooner, a cold-resistant wild tomato, were evaluated in the field. Four plantings were established between 21 August and 2 October 2006 (day 232 and 274). Throughout the experiment the heat sum was interpreted using air temperature to calculate growing degree days (base 12 °C) and chilling hours (below 12 °C), with the daily light integral calculated as global solar radiation. The relative post-transplant growth rate, fruit set and yield, were evaluated over two successive 10-d periods. Chilling tolerance...
Tipo: Journal article Palavras-chave: Linkage drag; NILs.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400004
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Bayesian Analysis of the Genetic Control of Survival in F3 Families of Common Bean Chilean J. Agric. Res.
Mora,Freddy; Gonçalves-Vidigal,Maria C; Santos,Alexandra I.
The objectives of this study were to examine the genetic control of survival in segregant families F3 of the common bean (Phaseolus vulgaris L.) in southern Brazil during the 2004-2005 growing season, to identify useful genotypes for the breeding program of this crop, and to determine the genetic association between survival and weight of 100 seeds (production trait; P100). A Bayesian approach was used to predict breeding values and to estimate variance components. Survival was recorded as a binary response: dead plant or live plant during harvest. The total population consisted of 11,520 individual plants. The difference in the magnitude between the best and the worst families was as high as 22%, and varied from 57 to 73%. Survival was found to be highly...
Tipo: Journal article Palavras-chave: Genetic improvement; Breeding; Gibbs algorithm; Heritability.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400003
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Genetic Relationships of Common Bean (Phaseolus vulgaris L.) Race Chile with Wild Andean and Mesoamerican Germplasm Chilean J. Agric. Res.
Becerra V,Viviana; Paredes C,Mario; Debouck,Daniel.
The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and...
Tipo: Journal article Palavras-chave: Chilean common bean germplasm; Chloroplast DNA; Mitochondrial DNA; PCR-RFLP.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100001
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Nitrogen Mineralization and Released Nutrients in a Volcanic Soil Amended with Poultry Litter Chilean J. Agric. Res.
Hirzel,Juan; Undurraga,Pablo; Walter,Ingrid.
Optimum application rates of poultry litter (PL) spread out on the farmer´s field is a valuable source of available plant nutrients. The aim of this study was to compare the effect of two rates of PL and conventional fertilization (CF) on N mineralization and P, K, Zn, and Cu availability in an Andisol from Southern Chile under controlled conditions. Aerobic incubation was carried out for a 16-wk period. N mineralization rates were higher (61.5%) with the two PL treatments than with conventional fertilizer (23%). CF was associated with high N availability prior to the start of incubation and slight immobilization during the first week, perhaps due to a more rapid conversion of urea into NH4 which was then temporarily immobilized by the microbial biomass....
Tipo: Journal article Palavras-chave: Aerobic incubation; Poultry litter; Nitrogen mineralization; Nutrient availability; Volcanic soil.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000100012
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