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Acute effect of different antidepressants on glycemia in diabetic and non-diabetic rats BJMBR
Gomez,R.; Huber,J.; Tombini,G.; Barros,H.M.T..
Diabetic patients have a 20% higher risk of depression than the general population. Treatment with antidepressant drugs can directly interfere with blood glucose levels or may interact with hypoglycemic agents. The treatment of depression in diabetic patients must take into account variations of glycemic levels at different times and a comparison of the available antidepressant agents is important. In the present study we evaluated the interference of antidepressants with blood glucose levels of diabetic and non-diabetic rats. In a first experiment, male adult Wistar rats were fasted for 12 h. Imipramine (5 mg/kg), moclobemide (30 mg/kg), clonazepam (0.25 mg/kg), fluoxetine (20 mg/kg) sertraline (30 mg/kg) or vehicle was administered. After 30 min, fasting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Depression; Antidepressants; Glucose tolerance; Diabetes; Glycemia.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100007
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Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women BJMBR
Cardoso,A.M.; Bagatini,M.D.; Roth,M.A.; Martins,C.C.; Rezer,J.F.P.; Mello,F.F.; Lopes,L.F.D.; Morsch,V.M.; Schetinger,M.R.C..
The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P < 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intermittent exercise; Anaerobic exercise; Oxidative stress; Immune suppression; Middle-aged women.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200010
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Acute effects of selective serotonin reuptake inhibitors on neuroleptic-induced catalepsy in mice BJMBR
Pires,J.G.P.; Bonikovski,V.; Futuro-Neto,H.A..
Depression found in Parkinson disease (PD) usually responds to selective serotonin reuptake inhibitors (SSRIs). Drugs that modify experimental neuroleptic catalepsy (NC) might affect extrapyramidal symptoms in PD. Therefore, the effects of SSRIs on NC were tested in mice, 26-36 g, separated by sex. Catalepsy was induced with haloperidol (H; 1 mg/kg, ip) and measured at 30-min intervals using a bar test. An SSRI (sertraline, ST; paroxetine, PX; fluoxetine) or vehicle (C) was injected ip 30 min before H. Dunnett's test was used for comparison of means. ST (1-5 mg/kg) or PX (1-5 mg/kg) attenuated NC, with a similar inhibition found in both sexes (5 mg/kg, 180 min: ST - males: 124 ± 10 vs 714 ± 15 s in C; females: 116 ± 10 vs 718 ± 6 s in C; PX - males: 106 ±...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Selective serotonin reuptake inhibitors; Fluoxetine; Sertraline; Paroxetine; Extrapyramidal system; Catalepsy.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200015
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Acute electrophysiologic consequences of pyridostigmine inhibition of cholinesterase in humans BJMBR
Zimerman,L.I.; Liberman,A.; Castro,R.R.T.; Ribeiro,J.P.; Nóbrega,A.C.L..
The cardiovascular electrophysiologic basis for the action of pyridostigmine, an acetylcholinesterase inhibitor, has not been investigated. The objective of the present study was to determine the cardiac electrophysiologic effects of a single dose of pyridostigmine bromide in an open-label, quasi-experimental protocol. Fifteen patients who had been indicated for diagnostic cardiac electrophysiologic study underwent two studies just before and 90-120 min after the oral administration of pyridostigmine (45 mg). Pyridostigmine was well tolerated by all patients. Wenckebach nodal anterograde atrioventricular point and basic cycle were not altered by pyridostigmine. Sinus recovery time (ms) was shorter during a 500-ms cycle stimulation (pre: 326 ± 45 vs post:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiac electrophysiology; Parasympathetic nervous system; Cholinesterase inhibitors; Cardiovascular disease; Pyridostigmine bromide; Autonomic nervous system.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200013
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Acute exercise inhibits gastric emptying of liquids in rats: influence of the NO-cGMP pathway BJMBR
Cavalcante,A.K.M.; Siqueira,R.C.L.; Feitosa Júnior,V.N.; de Andrade,C.R.; Santos,A.A.; Silva,M.T.B..
We previously found that acute exercise inhibited the gastric emptying of liquid in awake rats by causing an acid-base imbalance. In the present study, we investigated the involvement of the nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathway, vasoactive intestinal peptide (VIP), and corticotropin-releasing factor (CRF) peptide in this phenomenon. Male rats were divided into exercise or sedentary group and were subjected to a 15-min swim session against a load (2.5 or 5% b.w.). The rate of gastric emptying was evaluated after 5, 10, or 20 min postprandially. Separate groups of rats were treated with vehicle (0.9% NaCl, 0.1 mL/100 g, ip) or one of the following agents: atropine (1.0 mg/kg, ip), the NO non-selective inhibitor Nω-nitro-L-arginine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute exercise; Bioelectrical impedance; Gastric motility; High-intensity exercise.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100607
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Acute exercise performed close to the anaerobic threshold improves cognitive performance in elderly females BJMBR
Córdova,C.; Silva,V.C.; Moraes,C.F.; Simões,H.G.; Nóbrega,O.T..
The objective of the present study was to compare the effect of acute exercise performed at different intensities in relation to the anaerobic threshold (AT) on abilities requiring control of executive functions or alertness in physically active elderly females. Forty-eight physically active elderly females (63.8 ± 4.6 years old) were assigned to one of four groups by drawing lots: control group without exercise or trial groups with exercise performed at 60, 90, or 110% of AT (watts) and submitted to 5 cognitive tests before and after exercise. Following cognitive pretesting, an incremental cycle ergometer test was conducted to determine AT using a fixed blood lactate concentration of 3.5 mmol/L as cutoff. Acute exercise executed at 90% of AT resulted in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; Alert; Anaerobic threshold; Exercise; Executive functions; Response time test.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500010
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Acute exposure to lead increases myocardial contractility independent of hypertension development BJMBR
Fioresi,M.; Furieri,L.B.; Simões,M.R.; Ribeiro Junior,R.F.; Meira,E.F.; Fernandes,A.A.; Stefanon,I.; Vassallo,D.V..
We studied the effects of the acute administration of small doses of lead over time on hemodynamic parameters in anesthetized rats to determine if myocardial contractility changes are dependent or not on the development of hypertension. Male Wistar rats received 320 µg/kg lead acetateiv once, and their hemodynamic parameters were measured for 2 h. Cardiac contractility was evaluated in vitro using left ventricular papillary muscles as were Na+,K+-ATPase and myosin Ca2+-ATPase activities. Lead increased left- (control: 112 ± 3.7 vs lead: 129 ± 3.2 mmHg) and right-ventricular systolic pressures (control: 28 ± 1.2vs lead: 34 ± 1.2 mmHg) significantly without modifying heart rate. Papillary muscles were exposed to 8 µM lead acetate and evaluated 60 min later....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lead; Myocardial contractility; Ventricular pressure.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200178
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Acute extracellular fluid volume changes increase ileocolonic resistance to saline flow in anesthetized dogs BJMBR
Santiago Jr.,A.T.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; da-Graça,J.R.V.; de-Oliveira,G.R.; dos-Santos,A.A.; Rola,F.H..
We determined the effect of acute extracellular fluid volume changes on saline flow through 4 gut segments (ileocolonic, ileal, ileocolonic sphincter and proximal colon), perfused at constant pressure in anesthetized dogs. Two different experimental protocols were used: hypervolemia (iv saline infusion, 0.9% NaCl, 20 ml/min, volume up to 5% body weight) and controlled hemorrhage (up to a 50% drop in mean arterial pressure). Mean ileocolonic flow (N = 6) was gradually and significantly decreased during the expansion (17.1%, P<0.05) and expanded (44.9%, P<0.05) periods while mean ileal flow (N = 7) was significantly decreased only during the expanded period (38%, P<0.05). Mean colonic flow (N = 7) was decreased during expansion (12%, P<0.05) but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ileocolonic motility; Extracellular fluid volume; Expansion; Hemorrhage; Atropine.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800014
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Acute kidney injury in AIDS: frequency, RIFLE classification and outcome BJMBR
Libório,A.B.; Mota,R.M.S.; Abreu,K.L.S.; Silva,A.E.B.; Araújo,S.M.H.A.; Daher,E.F..
The objective of the present study was to evaluate the characteristics of acute kidney injury (AKI) in AIDS patients and the value of RIFLE classification for predicting outcome. The study was conducted on AIDS patients admitted to an infectious diseases hospital inBrazil. The patients with AKI were classified according to the RIFLE classification: R (risk), I (injury), F (failure), L (loss), and E (end-stage renal disease). Univariate and multivariate analyses were used to evaluate the factors associated with AKI. A total of 532 patients with a mean age of 35 ± 8.5 years were included in this study. AKI was observed in 37% of the cases. Patients were classified as "R" (18%), "I" (7.7%) and "F" (11%). Independent risk factors for AKI were thrombocytopenia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute kidney injury; HIV; AIDS; Prognosis; Mortality; RIFLE.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100013
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Acute lead-induced vasoconstriction in the vascular beds of isolated perfused rat tails is endothelium-dependent BJMBR
Silveira,E.A.; Lizardo,J.H.F.; Souza,L.P.; Stefanon,I.; Vassallo,D.V..
Chronic lead exposure induces hypertension in humans and animals, affecting endothelial function. However, studies concerning acute cardiovascular effects are lacking. We investigated the effects of acute administration of a high concentration of lead acetate (100 µΜ) on the pressor response to phenylephrine (PHE) in the tail vascular bed of male Wistar rats. Animals were anesthetized with sodium pentobarbital and heparinized. The tail artery was dissected and cannulated for drug infusion and mean perfusion pressure measurements. Endothelium and vascular smooth muscle relaxation were tested with acetylcholine (5 µg/100 µL) and sodium nitroprusside (0.1 µg/100 µL), respectively, in arteries precontracted with 0.1 µM PHE. Concentration-response curves to PHE...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lead acetate; Vascular reactivity; Endothelium; Hypertension.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500011
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Acute leukemia in early childhood BJMBR
Emerenciano,M.; Koifman,S.; Pombo-de-Oliveira,M.S..
Acute leukemia in early childhood is biologically and clinically distinct. The particular characteristics of this malignancy diagnosed during the first months of life have provided remarkable insights into the etiology of the disease. The pro-B, CD10 negative immunophenotype is typically found in infant acute leukemia, and the most common genetic alterations are the rearrangements of the MLL gene. In addition, the TEL/AML1 fusion gene is most frequently found in children older than 24 months. A molecular study on a Brazilian cohort (age range 0-23 months) has detected TEL/AML1+ve (N = 9), E2A/PBX1+ve (N = 4), PML/RARA+ve (N = 4), and AML1/ETO+ve (N = 2) cases. Undoubtedly, the great majority of genetic events occurring in these patients arise prenatally....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infant acute leukemia; MLL; Acute lymphoblastic leukemia; Acute myeloid leukemia; Maternal exposures; Molecular and exploratory epidemiology.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600002
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Acute leukemias in Piauí: comparison with features observed in other regions of Brazil BJMBR
Rego,M.F.N.; Pinheiro,G.S.; Metze,K.; Lorand-Metze,I..
Differences in age and sex distribution as well as FAB (French-American-British classification) types have been reported for acute leukemias in several countries. We studied the demographics and response to treatment of patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) between 1989 and 2000 in Teresina, Piauí, and compared these results with reports from Brazil and other countries. Complete data concerning 345 patients (230 ALL, 115 AML) were reviewed. AML occurred predominantly in adults (77%), with a median age of 34 years, similar to that found in the southeast of Brazil but lower than the median age in the United States and Europe (52 years). FAB distribution was similar in children and adults and FAB-M2 was the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute lymphoblastic leukemia; Acute myeloid leukemia; Epidemiology.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300007
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Acute myeloid leukemia in elderly patients: experience of a single center BJMBR
Rodrigues,C.A.; Chauffaille,M.L.L.F.; Pelloso,L.A.F.; Ghaname,F.S.; Kerbauy,D.M.B.; Campos,M.G.V.; Yamamoto,M..
Acute myeloid leukemia (AML) is a disease predominantly of older adults. Treatment of AML in the elderly is complicated not only by comorbidities but also by the high prevalence of poor prognosis markers. Thirty-one consecutive unselected patients with AML older than 60 years (representing 33% of all AML cases diagnosed at our institution during the same period) were followed over a period of 5 years (1997-2002). A high incidence of AML with multilineage dysplasia (45%) and no favorable cytogenetic abnormalities but 62% intermediate and 38% unfavorable karyotypes were found. Sixteen patients (52%) were selected for induction of intensive cytotoxic treatment and complete remission was achieved only by some of these intensively treated patients (7 of 16). Of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute myeloid leukemia; Elderly patients; G-banding karyotype; Induction therapy; Overall survival.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600004
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Acute phenobarbital administration induces hyperalgesia: pharmacological evidence for the involvement of supraspinal GABA-A receptors BJMBR
Yokoro,C.M.; Pesquero,S.M.S.; Turchetti-Maia,R.M.M.; Francischi,J.N.; Tatsuo,M.A.K.F..
The aim of the present study was to determine if phenobarbital affects the nociception threshold. Systemic (1-20 mg/kg) phenobarbital administration dose dependently induced hyperalgesia in the tail-flick, hot-plate and formalin tests in rats and in the abdominal constriction test in mice. Formalin and abdominal constriction tests were the most sensitive procedures for the detection of hyperalgesia in response to phenobarbital compared with the tail-flick and hot-plate tests. The hyperalgesia induced by systemic phenobarbital was blocked by previous administration of 1 mg/kg ip picrotoxin or either 1-2 mg/kg sc or 10 ng icv bicuculline. Intracerebroventricular phenobarbital administration (5 µg) induced hyperalgesia in the tail-flick test. In contrast,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenobarbital; Hyperalgesia; GABA-A receptors.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300015
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Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques BJMBR
Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J..
Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean:
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006
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Acute stress-induced antinociception is cGMP-dependent but heme oxygenase-independent BJMBR
Carvalho-Costa,P.G.; Branco,L.G.S.; Leite-Panissi,C.R.A..
Endogenous carbon monoxide (CO), which is produced by the enzyme heme oxygenase (HO), participates as a neuromodulator in physiological processes such as thermoregulation and nociception by stimulating the formation of 3′,5′-cyclic guanosine monophosphate (cGMP). In particular, the acute physical restraint-induced fever of rats can be blocked by inhibiting the enzyme HO. A previous study reported that the HO-CO-cGMP pathway plays a key phasic antinociceptive role in modulating noninflammatory acute pain. Thus, this study evaluated the involvement of the HO-CO-cGMP pathway in antinociception induced by acute stress in male Wistar rats (250-300 g; n=8/group) using the analgesia index (AI) in the tail flick test. The results showed that antinociception...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heme oxygenase; Carbon monoxide; CGMP; Lateral cerebral ventricle; Acute stress; Antinociception.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201057
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Adaptive changes in thyroid function of female rats fed a high-fat and low-protein diet during gestation and lactation BJMBR
Brito,P.D.; Ramos,C.F.; Passos,M.C.F.; Moura,E.G..
Tipo: Info:eu-repo/semantics/article Palavras-chave: High-lipid diet; Low-protein diet; Thyroid function; Lactation; Gestation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600015
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Addressing conflicts of interest in the research paper: a societal demand in contemporary science? BJMBR
Vasconcelos,S.M.R.; Cassimiro,M.C.; Martins,M.F.M.; Palácios,M..
In the last decade, dialogue between science and society has found a forum in an increasing number of publications on topics such as public engagement with science and public trust in science. Concerning the latter, issues that include cases of research misconduct, accountability in research, and conflicts of interest (COIs) have shaped global discussions on the communication of science. In the publication setting, the perception that hiding COIs and/or not managing them well may affect public trust in the research record has grown among editors. We conducted a search for editorials addressing COIs between 1989 and 2011, using four major databases: Medline/PubMed, Embase, Scopus, and Web of Knowledge. We explored the content of these editorials and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conflicts of interest; Contemporary science; Science policy; Science education; Research integrity; Science and society.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201007
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Adeno-associated virus for cystic fibrosis gene therapy BJMBR
Martini,S.V.; Rocco,P.R.M.; Morales,M.M..
Gene therapy is an alternative treatment for genetic lung disease, especially monogenic disorders such as cystic fibrosis. Cystic fibrosis is a severe autosomal recessive disease affecting one in 2500 live births in the white population, caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR). The disease is classically characterized by pancreatic enzyme insufficiency, an increased concentration of chloride in sweat, and varying severity of chronic obstructive lung disease. Currently, the greatest challenge for gene therapy is finding an ideal vector to deliver the transgene (CFTR) to the affected organ (lung). Adeno-associated virus is the most promising viral vector system for the treatment of respiratory disease because it...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene therapy; Adeno-associated virus; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Vectors.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100004
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Adenosine A1 receptor-mediated inhibition of in vitro prolactin secretion from the rat anterior pituitary BJMBR
Picanço-Diniz,D.L.W.; Valença,M.M.; Antunes-Rodrigues,J..
In previous studies, we demonstrated biphasic purinergic effects on prolactin (PRL) secretion stimulated by an adenosine A2 agonist. In the present study, we investigated the role of the activation of adenosine A1 receptors by (R)-N6-(2-phenylisopropyl)adenosine (R-PIA) at the pituitary level in in vitro PRL secretion. Hemipituitaries (one per cuvette in five replicates) from adult male rats were incubated. Administration of R-PIA (0.001, 0.01, 0.1, 1, and 10 µM) induced a reduction of PRL secretion into the medium in a U-shaped dose-response curve. The maximal reduction was obtained with 0.1 µM R-PIA (mean ± SEM, 36.01 ± 5.53 ng/mg tissue weight (t.w.)) treatment compared to control (264.56 ± 15.46 ng/mg t.w.). R-PIA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclopentyltheophylline; Dibutyryl cAMP; R-PIA; Pertussis toxin; Cholera toxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100013
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