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Registros recuperados: 12
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Insecticidal and antifungic proteins of the latex from Manihot glaziovii Muell. Arg. Rev. Bras. Bot.
PEREIRA,LUCILENE S.; GOMES,VALDIRENE M.; FERNANDES,KÁTIA V. S.; SALES,MAURÍCIO P.; XAVIER-FILHO,JOSÉ.
Analysis of the latex from Manihot glaziovii showed the presence of various enzymatic and inhibitory activities. The latex also presented an inhibitory effect on the development of cowpea weevil (Callosobruchus maculatus) in an artificial seed system and on the development, in an in vitro assay, of phytopathogenic fungi Colletotrichum gloesporioides, Fusarium solani and Macrophomina phaseolina. These results suggest the presence of substances, some of them of protein nature, involved in plant defense mechanisms in this exudation product.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Latex chitinase <; FONT FACE=Symbol>; B<; /FONT>; -1 3-glucanase proteinase inhibitors defense proteins.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000100005
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Estimação de parâmetros genéticos em bovinos de corte utilizando os métodos de máxima verossimilhança restrita e  R. Bras. Zootec.
Melis,Marcelo Hessel Van; Eler,Joanir Pereira; Silva,Josineudson Augusto II de Vasconcelos; Ferraz,José Bento Sterman.
Com o objetivo de comparar as estimativas de parâmetros genéticos obtidas pelos métodos de máxima verossimilhança restrita (REML) e <FONT FACE=Symbol>Â</FONT>, foram realizadas análises uni-características sob modelo animal, para os quais foi utilizado um conjunto de dados da raça Nelore. Os modelos estatísticos incluíram os efeitos fixos de grupo de contemporâneos (fazenda, ano de nascimento, sexo e grupos de manejo à desmama e ao sobreano) e idade do animal (covariável linear), e como aleatórios foram considerados os efeitos genético aditivo direto e residual. As estimativas dos coeficientes de herdabilidade foram 0,36 e 0,39 para peso aos 18 meses (PESSOB); 0,27 e 0,29 para ganho de peso da desmama aos 18 meses (GP345); 0,22 e 0,21 para...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Características de crescimento escore visual Método <; FONT FACE=Symbol>; Â<; /FONT>; Nelore parâmetros genéticos REML.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000700011
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Estimação de parâmetros genéticos para probabilidade de prenhez aos 14 meses e altura na garupa em bovinos da raça Nelore R. Bras. Zootec.
Silva,Josineudson Augusto II de Vasconcelos; Van Melis,Marcelo Hessel; Eler,Joanir Pereira; Ferraz,José Bento Sterman.
Registros de 24.703 animais da raça Nelore, provenientes de seis fazendas, foram utilizados para estimar os coeficientes de herdabilidade e a correlação genética relativos às características probabilidade de prenhez aos 14 meses (PP14) e altura na garupa aos 450 dias de idade (AG450). O modelo matemático incluiu os efeitos fixos de grupo de contemporâneo (181 grupos) e classe de idade da mãe ao parto (7 classes) para PP14 e o efeito fixo de grupo de contemporâneo (584 grupos) para AG450. Os efeitos aleatórios incluídos em ambos os modelos foram genético aditivo do touro e residual. Os componentes de variância e covariância foram obtidos pelo método <FONT FACE=Symbol>Â</FONT>. As estimativas de herdabilidade foram iguais a 0,73 ± 0,01 e 0,30 ±...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Altura na garupa correlação genética herdabilidade método <; FONT FACE=Symbol>; Â<; /FONT>; Prenhez de novilha.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000500014
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Production of amylases by Aspergillus tamarii Rev. Microbiol.
Moreira,Fabiana Guillen; Lima,Francieli Arrias de; Pedrinho,Sophia Renata Fazzano; Lenartovicz,Veridiana; Souza,Cristina Giatti Marques de; Peralta,Rosane Marina.
A strain of Aspergillus tamarii, a filamentous fungus isolated from soil, was able to produce both <FONT FACE="Symbol">a</FONT>-amylase and glucoamylase activities in mineral media supplemented with 1% (w/v) starch or maltose as the carbon source. Static cultivation led to significantly higher yields than those obtained using shaking culture. The production of amylases was tolerant to a wide range of initial culture pH values (from 4 to 10) and temperature (from 25 to 42oC). Two amylases, one <FONT FACE="Symbol">a</FONT>-amylase and one glucoamylase, were separated by ion exchange chromatography. Both partially purified enzymes had optimal activities at pH values between 4.5 and 6.0 and were stable under acid conditions (pH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: <; FONT FACE=Symbol>; A<; /FONT>; -amylase Aspergillus tamarii glucoamylase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200014
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Production of a-amylase in acid cheese whey culture media with automatic pH control Rev. Microbiol.
Ferreyra,Rosana; Lorda,Graciela; Balatti,Antonio.
The influence of aeration and automatic pH control on the production of <FONT FACE="Symbol">a</FONT>-amylase by a strain of Bacillus subtilis NRRL 3411 from acid cheese whey was studied. Tests were carried out in a rotary shaker and in mechanically stirred fermenters. <FONT FACE="Symbol">a</FONT>-amylase was analysed according to DUN’s method. Oxygen absorption rate was determined by Cooper’s method. Cell oxygen demand was determined as oxygen consumption in a Warburg respirometer. The level of dissolved oxygen was measured by means of a galvanic silver-lead electrode. Results suggest the possibility of industrial use of acid cheese whey as a carbon source for <FONT FACE="Symbol">a</FONT>-amylase production, since the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis <; FONT FACE=Symbol>; A<; /FONT>; -amylase acid cheese whey automatic pH control.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400005
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INTERFERON SELECTIVELY INHIBITS THE SYNTHESIS OF MAYARO VIRUS GLYCOPROTEINS Rev. Microbiol.
Ferreira,Davis F.; Rebello,Maria Christina Soares.
We have previously observed that interferon (recIFN<FONT FACE="Symbol">a</FONT>2b) blocks the process of morphogenesis of Mayaro virus in TC7 cells (monkey kidney). In this work we show that IFNa inhibits preferentially virus glycoproteins and their precursors, and this effect is probably correlated to the alterations in the morphogenesis process previously observed.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Interferon <; FONT FACE=Symbol>; A<; /FONT>; Mayaro virus glycoproteins morphogenesis.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300014
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Antioxidant activity of the microalga Spirulina maxima BJMBR
Miranda,M.S.; Cintra,R.G.; Barros,S.B.M.; Mancini-Filho,J..
Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation) of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Microalgae antioxidant activity Spirulina maxima phenolic compounds ß-carotene <; FONT FACE=Symbol>; A<; /FONT>; -tocopherol.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800007
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Regulation of T cell response to leishmania antigens by determinants of histocompatibility leukocyte class I and II molecules BJMBR
Bacellar,O.; Russo,C.; Carvalho,E.M..
It has been shown that HLA class I molecules play a significant role in the regulation of the proliferation of T cells activated by mitogens and antigens. We evaluated the ability of mAb to a framework determinant of HLA class I molecules to regulate T cell proliferation and interferon gamma (IFN-<FONT FACE="Symbol">g</FONT>) production against leishmania, PPD, C. albicans and tetanus toxoid antigens in patients with tegumentary leishmaniasis and healthy subjects. The anti-major histocompatibility complex (MHC) mAb (W6/32) suppressed lymphocyte proliferation by 90% in cultures stimulated with <FONT FACE="Symbol">a</FONT>CD3, but the suppression was variable in cultures stimulated with leishmania antigen. This suppression ranged from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HLA class I tegumentary leishmaniasis modulation of T cell response by HLA class I interferon-<; FONT FACE=Symbol>; G<; /FONT>; And HLA class I soluble antigen and HLA class I.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200010
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The effect of methotrexate and azathioprine on the serum levels of IgA-a1-antitrypsin complex in juvenile chronic arthritis BJMBR
In the present study we investigated the influence of methotrexate (MTX) and azathioprine (AZA) on the serum levels of the IgA-<FONT FACE="Symbol">a</FONT>1-antitrypsin (IgA-AT) complex in patients with the systemic form of juvenile chronic arthritis (JCA). Fifty-six JCA patients (22 treated with MTX, 18 treated with AZA, and 16 not treated with any immunosuppressive agent) were enrolled in the study. MTX dosage ranged from 0.3 to 0.5 mg kg-1 week-1, while AZA was given daily at an average dose of 1 mg/kg. MTX was given for 13 months (SD = 7 months) whereas AZA for 11 months (SD = 6 months). The average value of the complex was higher in JCA patients than in both control groups (0.74 ± 0.73 U vs 0.37 ± 0.13 U (control children), P&lt;0.001...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Juvenile chronic arthritis IgA-<; FONT FACE=Symbol>; A<; /FONT>; 1-antitrypsin complex methotrexate azathioprine C-reactive protein <; FONT FACE=Symbol>; A<; /FONT>; 1-acid-glycoprotein <; FONT FACE=Symbol>; A<; /FONT>; 1-antichymotrypsin.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600010
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Topical application of a melanotropin analogue to vulgar vitiligo dermo-epidermal minigrafts BJMBR
Schwartzmann-Solon,A.M.; Visconti,M.A.; Castrucci,A.M.L..
Human subjects with active vulgar vitiligo do not respond well to autologous dermo-epidermal minigrafting. Eighteen subjects were treated with the <FONT FACE="Symbol">a</FONT>-melanocyte-stimulating hormone (<FONT FACE="Symbol">a</FONT>-MSH) synthetic analogue &amp;#091;Nle4, D-Phe7&amp;#093;-<FONT FACE="Symbol">a</FONT>-MSH. The hormone (50 µl, 0.4 mM) was applied topically to 30-cm2 lesions in which 29-48 minigrafts had been made. The hormone did not improve the success of the minigrafting and no differences were observed in local or distant repigmentation in treated subjects as compared to the placebo group. Aliquots of 24-h urine concentrated by lyophilization irreversibly darkened toad skins, demonstrating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vulgar vitiligo <; FONT FACE=Symbol>; A<; /FONT>; -MSH synthetic analogue minigrafting.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200008
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Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice BJMBR
Ferreira,A.P.; Aarestrup,F.M.; Bonecini-Almeida,M.G.; Souza,E.E.; Gomes,E.A.; Corrêa,J.O.A.; Teixeira,H.C..
Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57Bl/6 mice. C57Bl/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-<FONT FACE="Symbol">g</FONT>-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis Ascaris suum macrophage activation TNF-<; FONT FACE=Symbol>; A<; /FONT>; NADPH diaphorase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100014
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Performance of the Albicans ID2Ò chromogenic medium for rapid identification of Candida albicans BJM
Godoy-Martínez,Patricio; Azevedo,Ana C.; Reis,Viviane; Alves,Thelma; Almeida,Leila P.; Colombo,Arnaldo L..
The aim of our study was to evaluate the accuracy of the chromogenic media Albicans ID2<FONT FACE=Symbol>Ò</FONT> (bioMérieux, France) for the identification of Candida albicans among 330 yeast strains. All C. albicans (100) and C. dubliniensis (20) strains exhibited blue color when cultured on Albicans ID2<FONT FACE=Symbol>Ò</FONT>. However, the blue color was also exhibited by cultures of C. rugosa (30/30) and C. tropicalis (3/50) isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans chromogenic medium Albicans ID2<; FONT FACE=Symbol>; Ò<; /FONT>; Media.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300004
Registros recuperados: 12
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