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	Provedor de dados 58 (2) Autor Aguilar,C.N. (1) Castillo,Boris Gutarra (1) Fernández,Rubén Cuervo (1) Maiorano,Alfredo Eduardo (1) Maresma,Beatriz Guilarte (1) Martínez-Hernández,J.L. (1) Michelena,G. (1) Rodrigues,Maria Filomena de Andrade (1) Rodríguez-Herrera,R. (1) Silva,Elda Sabino da (1) Veana,F. (1) Mais... Palavra-chave Β-fructofuranosidase (2) Aspergillus oryzae (1) By-products (1) Fructooligosaccharides (1) Fructosyltransferase (1) Mutagenesis (1) UV irradiation (1) Virgin and hydrolyzated bagasse (1) Xerophilic (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1 58 Veana,F.; Martínez-Hernández,J.L.; Aguilar,C.N.; Rodríguez-Herrera,R.; Michelena,G.. Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previously reported as an invertase over-producer strain was used. Molasses from Mexico and Cuba were chemically analyzed (total and reducer sugars, nitrogen and phosphorous contents); the last one was selected based on chemical composition. Fermentations were performed using virgin and hydrolyzate bagasse (treatment with concentrated sulfuric acid). Results indicated that,... Tipo: Info:eu-repo/semantics/article Palavras-chave: By-products; Virgin and hydrolyzated bagasse; Β-fructofuranosidase; Xerophilic. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200002 Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase 58 Maresma,Beatriz Guilarte; Castillo,Boris Gutarra; Fernández,Rubén Cuervo; Silva,Elda Sabino da; Maiorano,Alfredo Eduardo; Rodrigues,Maria Filomena de Andrade. Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dish as the enzyme source. Seven mutants with fructofuranosidase activity values relative to the parent culture between 140 -190% were selected from survivors grown at temperature of 40ºC or 0.018% (w/v) sodium dodecyl sulfate concentration. They were cultivated on a rotary shaker to characterize mycelium and extracellular fructosyltransferase activities. Three mutants named IPT-745, IPT-746 and IPT-748... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus oryzae; Mutagenesis; Β-fructofuranosidase; Fructosyltransferase; Fructooligosaccharides; UV irradiation. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100027 Registros recuperados: 2 Primeira ... 1 ... Última

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