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| Kulchana Ketsuwan. |
| Next Generation Sequencing (NGS): SOLiD System Sequencing Platform was applied to investigate the whole transcriptome of two hybrids (PTT06001H; H1 and PTT06008H: H2), two paternal lines (CK81; P1 and CK120; P2), and maternal line (IR79156; M). The data sets were aligned against the reference, precisely, the total numbers of the individual rice panicles sample aligned with Nipponbare ranking from the highest were as follows, P2 (9,747,077 reads), H1 (7,386,061 reads) , P1 (5,361,077 reads) , H2 (5,196,982 reads), and M (2,244,306 reads), respectively. The differentially expressed genes (DEGs) were identified and found that 2902, 1604, 3168, 3068, 1171, and 3031 genes were up-regulated (> 2 fold) between M vs. H1, P1 vs. H1, M vs.P1, M vs. H2, P2 vs. H2,... |
| Tipo: PhysicalObject |
Palavras-chave: Hybrid rice; Heterosis; Differentially expressed genes; DEGs; Genetic transformation; Gene expression; Transcription; Nucleotide analysis; Plant breeding; Rice productivity; ข้าว; พันธุ์ลูกผสม; ช่อดอก; การถ่ายทอดทางพันธุกรรม; การแสดงออกของยีน; การถอดรหัสพันธุกรรม; การวิเคราะห์นิวคลีโอไทด์; การปรับปรุงพันธุ์; ศักยภาพความดีเด่นเหนือพ่อแม่; การเพิ่มผลผลิต. |
| Ano: 2014 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5659 |
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| Kulchana Ketsuwan. |
| Next Generation Sequencing (NGS): SOLiD System Sequencing Platform was applied to investigate the whole transcriptome of two hybrids (PTT06001H; H1 and PTT06008H: H2), two paternal lines (CK81; P1 and CK120; P2), and one maternal line (IR79156; M). The data sets were aligned against the reference, precisely, the total numbers of the individual rice panicles. Samples aligned with Nipponbare ranking from the highest were as follows, P2 (9,747,077 reads), H1 (7,386,061 reads), P1 (5,361,077 reads), H2 (5,196,982 reads), and M (2,244,306 reads), respectively. The differentially expressed genes (DEGs) were identified and found that 2902, 1604, 3168, 3068, 1171, and 3031 genes were up-regulated (> 2 fold) between M vs. H1, P1 vs. H1, M vs.P1, M vs. H2, P2 vs.... |
| Tipo: Collection |
Palavras-chave: Hybrid rice Transcriptome Gene expression Heterosis ข้าวลูกผสม; การถอดรหัสพันธุกรรม; การแสดงออกของยีน; ความดีเด่นเหนือพ่อแม่. |
| Ano: 2014 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5942 |
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| Siriphorn Rotarayanont; Pawini Dankittikool; Chutima Saesea; Bhutri Sornboon; Tawatchai Wichayapol; Jaruwan Oonnankart. |
| SSSIVb gene, which is the group of Soluble Starch Synthase enzyme, concerns to synthesis amylopectin in the seed of rice. In this research, we will examine the process of mRNA splicing intron 6, 7, 8 and 9 of SSSIVb gene and observe the expression of gene in development of seed at 5, 10 and 15 days after flowering (DAF) in 5 varieties of the rice which are RD10, Chai Nat 1, Niew Phrae 1, San-pah-tawng 1 and Suphan Buri 60. All of those varieties have different quality of cooking. From the experimental, we found that Chai Nat 1 had splicing in 5 DAF rice seeds but no expression in 10 and 15 DAF rice seeds. In Suphan Buri 60 had splicing in 10 DAF rice seeds but no expression in 5 and 15 DAF rice seeds. And RD 10, Niew Phrae 1, San Pah Tawng 1 had splicing... |
| Tipo: Collection |
Palavras-chave: Rice; MRNA; Splicing; Starch synthase gene; Amylopectin; Cooking quality; ข้าว; MRNA; การตัดต่อยีน; ยีนสร้างแป้ง; อมิโลเพคติน; คุณภาพการหุงต้ม; การแสดงออกของยีน. |
| Ano: 2012 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5351 |
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| Tatsaporn Siripug; Suporn Katawatin. |
| HSP70-2 is well accepted as an indicator of heat stress. The purpose of this study was to investigate the physiological responses to heat stress of differential genotypes of HSP70-2 in dairy cattle. Thirty two female Holstein Friesian (HF) crossbred heifers, 2-3 years of age, 266 ± 5.6 kg body weight were placed outdoor with partial roof. Blood samples were collected to do genotyping of HSP70-2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Ambient temperature, relative humidity and physiological responses, including rectal temperature, respiration rate, sweating rate, pulse rate, skin temperature, and N/L ratio were examined at 06.00, 14.00, 18.00 and 22.00. Genotyping revealed that there were 14 and 18... |
| Tipo: PhysicalObject |
Palavras-chave: Dairy cattle; Genotypes; Genetic variation; Hsp70-2; Heat stress; Heat tolerant; Genetic improvement; โคนม; รูปแบบจีโนไทป์; พันธุกรรม; การแสดงออกของยีน; ความแตกต่างทางพันธุกรรม; การตอบสนองทางสรีรวิทยา; ความเครียด; อากาศร้อน; การทนร้อน; การรักษาสมดุลความร้อน; การปรับปรุงพันธุ์. |
| Ano: 2014 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5750 |
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