| Crosses were made between waxy corn and quality protein corn as female and male parents, respectively. In segregating progenies of two crosses, Kwi no.1 x PopII no.53 and Kwi no.9 x PopII no.53, selfing and backcrossing methods were used and then marker-assisted selection of opaque-2 gene (o2o2) by phi057 and umc1066 and of waxy (wxwx) by phi022 and phi061 were applied to obtain the opaque-waxy corn (o2o2wxwx). For the first step of MAS, phi057 and umc1066 were employed to detect the opaque-2 gene (o2o2) in both of BC1S1 and S1 generations. The genotypic ratio of opaque-2 gene (O2O2:O2o2:o2o2) was 37:46:31 for BC1S1 and 42:146:42 for S1 from the cross of Kwi no.1 x PopII no.53 while it was 41:51:41 for BC1S1 and 41:169:35 for S1 from the cross of Kwi no.9... |
