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	Provedor de dados Thai Agricultural (3) Autor Kasem Soontrajarn (2) Marsuton Sanyapeung (2) Nittaya Phromwong (2) Somsong Chotechuen (2) Payorm Cobelli (1) Poonsak Mekwatanakarn (1) Roongnapa Kawichai (1) Varapong Chamarerk (1) Mais... Palavra-chave Real-time PCR (3) ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง (3) Fragrant gene (2) ยีนควบคุมความหอม (2) Aromatic rice (1) Bt63 (1) GM rice (1) Genotype (1) HRM analysis (1) High Resolutions Melting analysis (1) High resolutions melting analysis (1) Rice varieties detect (1) การตรวจสอบยีน (1) การวิเคราะห์ HRM (1) ข้าว (1) ข้าวดัดแปรพันธุกรรม (1) ข้าวหอม (1) ศูนย์ปฏิบัติการและเก็บเมล็ดเชื้อพันธุ์ข้าวแห่งชาติ (1) เมล็ดพันธุ์ (1) Mais... Tipo do documento PhysicalObject (2) Collection (1) Ano 2015 (1) 2013 (2) País Thailand (3) Idioma Thailandês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última การตรวจสอบยีนควบคุมความหอมของข้าวด้วยวิธีปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง Thai Agricultural Kasem Soontrajarn; Marsuton Sanyapeung; Nittaya Phromwong; Somsong Chotechuen. Aromatic rice is a specific group of rice having high price in the world rice market. Fragrant trait is therefore included in the principal goal for rice production and improvement. A recessive gene on chromosome 8 has been elucidated to be homology with the gene encoded for betaine aldehyde dehydrogenase (BADH2). In fragrant rice this gene was mutated by 8 bp deletion in exon 7 resulting in accumulation of 2-acetyl-1-pyrroline (2AP). In this study, BADEX7-5 primer which is specific for fragrant gene (badh2) was tested by conventional PCR and Real-time PCR. Both methods showed the same PCR product size of 95 and 103 bp in fragrant and non-fragrant rice, respectively. High Resolutions Melting analysis demonstrated that the fragrant and non-fragrant... Tipo: PhysicalObject Palavras-chave: Fragrant gene; Real-time PCR; High resolutions melting analysis; Aromatic rice; Rice varieties detect; HRM analysis; Genotype; ยีนควบคุมความหอม; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง; ข้าวหอม; เมล็ดพันธุ์; การตรวจสอบยีน; ศูนย์ปฏิบัติการและเก็บเมล็ดเชื้อพันธุ์ข้าวแห่งชาติ. Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5685 การตรวจวิเคราะห์ข้าวและสินค้าข้าวดัดแปรพันธุกรรมด้วยปฏิกิริยาลูกโซ่ ในสภาพเวลาจริง Thai Agricultural Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn. Genetically modified (GM) insecticidal rice line, Bt63 (Shanyou63), has been developed in China and found unauthorized in processed rice products in many countries. This transgenic rice contains a copy of the synthetic DNA sequence with two genes: CryIA(b) and CryIA(c). These genes encode for the respective Bt toxins which is lethal to Lepidoptera. The aim of this study was to determine the contamination of Bt63 in non-labelled commercially available processed rice products from supermarkets. In this study, a real-time Polymerase Chain Reaction (PCR) was used to simultaneously determine the contamination of Bt63. The survey was conducted on a total of 21 and 42 non-labeled samples of processed rice products collected in the years 2011 and 2012,... Tipo: Collection Palavras-chave: GM rice; Bt63; Real-time PCR; ข้าวดัดแปรพันธุกรรม; Bt63; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง. Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5923 การตรวจสอบยีนควบคุมความหอมของข้าว ด้วยวิธีปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง Thai Agricultural Kasem Soontrajarn; Marsuton Sanyapeung; Nittaya Phromwong; Somsong Chotechuen. Aromatic rice is a specific group of rice having high price in the world rice market. A fragrant trait is therefore included in the principal goal for rice production and improvement. A recessive gene on chromosome 8 has been elucidated to be homology with the gene encoded for betaine aldehyde dehydrogenase (BADH2). In fragrant rice this gene was mutated by 8 bp deletion in exon 7 resulting in accumulation of 2-acetyl-1-pyrroline (2AP). In this study, the BADEX7-5 primer which is specific for the fragrant gene (badh2) was tested by a conventional PCR and a Real-time PCR. Both methods showed the same PCR product size of 95 and 103 bp in fragrant and non-fragrant rice, respectively. DNA sequencing and nucleotide alignment of those PCR products showed that... Tipo: PhysicalObject Palavras-chave: Fragrant gene; Real-time PCR; High Resolutions Melting analysis; ข้าว; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง; การวิเคราะห์ HRM; ยีนควบคุมความหอม. Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5529 Registros recuperados: 3 Primeira ... 1 ... Última

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