| Study was conducted to test find out the efficiency of the antagonistic bacterium Bacillus subtilis B006 against to Botryosphaeria rhodina cause of gummosis disease of cucurbit. The bacterium was determined its inhibition capability on the growth of B. rhodina 14 isolates. The results revealed that the antagonistic capability was significantly different into 9 groups. This bacterium was cultured in nutrient broth (NB) added 2% glucose and incubated at 28°C for 3, 5, 7 and 9 days and then, treated at 80°C for 30 min for heat tolerance cells. The maximum heat tolerant cells production (46-47%) was obtained 5-7 days of incubation. Hence the antagonistic bacterium B006 was cultured on such medium and temperature for 5 days and used to incorporate into four... |
