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Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing 69
Li,Meng Jun; Li,Ya Qing; Zhang,Nan; Shi,Zhan Liang.
Background: Wheat 1BL/1RS translocation lines are planted around the world for their disease resistance and high yield. Most of them are poor in bread making, which is partially caused by ω-secalins that are encoded by the ω-secalin gene family, which is located on the short arm of rye chromosome 1R (1RS). However, information on the structure and evolution of the ω-secalin gene family is still limited. Results: We first generated a physical map of the ω-secalin gene family covering 195 kb of the Sec-1 locus based on sequencing three bacterial artificial chromosome (BAC) clones of the 1BL/1RS translocation wheat cultivar Shimai 15. A BAC contig was constructed spanning 168 kb of the Sec-1 locus on 1RS. Twelve...
Tipo: Journal article Palavras-chave: Bacterial artificial chromosome (BAC) Wheat ω -Secalin 1BL/1RS translocation line.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300010
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