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Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis Phyton
Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
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