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| Chen,Liang; Xia,Hengchuan; Wang,Yiting; Chen,Keping; Qin,Lvgao; Wang,Bin; Yao,Qin; Li,Jun; He,Yuanqing; Zhao,Ermi. |
| Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2D electrophoresis; Elaphe taeniura; MALDI-TOF mass spectrometry; Protein. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020 |
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