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Selection of 5-fluorocytosine-resistant mutants from an Aspergillus niger citric acid-producing strain BJM
Conte,Ana Paula de F.; Marin,José M..
Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to 5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental strain.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Citric acid; 5-fluorocytosine.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100001
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Reversion by calcium of a yeast-like development to the original filamentous form, of the 10V10 5-fluorocytosine-sensitive mutant of Aspergillus niger BJM
Goulart,Rosangela de Carvalho; Marin,José Moacir.
Some filamentous fungi present the phenomenon of dimorphism, their morphological structure alterations being capable of inducing metabolism changes. The Aspergillus niger strain 10v10, a producer of citric acid, was submitted to the mutagenic action of ultraviolet irradiation which respectively selects mutants sensitive or resistant to the antifungi agent 5 fluorocytosine (5-FC). 5-FC sensitive mutants presented a morphological alteration to a yeast-like form. The effects of pH changes, addition of salts (KH2PO4, NH4NO3, MgSO4 and MgCl2), the presence of osmotic stabilizers, as well as of calcium chloride, on morphological reversal and acid production were studied. Morphological reversal to the filamentous form was observed only in the presence of CaCl2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; 5-fluorocytosine; Yeast-like; Calcium; Acid production; Dimorphism.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300018
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