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| Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Troni, Allan Reis; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Takamura, Angela Emi; Universidade Federal de Viçosa. |
| By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling... |
| Tipo: Estudos morfológicos |
Palavras-chave: 2.04.06.00-2; 5.05.04.00-2; 5.06.03.00-0 creole frog; GnRHa; Semen; Spermatozoon; Sperm motility Aquicultura. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
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| Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa.; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Seixas Filho, Jose Teixeira; Fundação Instituto de Pesca do Rio de Janeiro; Ribeiro, Christiano Demétrio de Lima; Universidade Federal de Viçosa. |
| Evaluation of semen characteristics after hormonal induction of the bullfrog could provide valuable information on the gametes of this species, which may be useful for projects related to artificial fertilization, animal improvement, and cryopreservation. Bullfrog males were induced to spermiate with buserelin acetate (GnRHa), and their semen was subsequently analyzed. GnRHa (0.4 μg) was administered to the bullfrog males with secondary sexual characteristics such as weight > 200 g, yellow chin, nuptial callus, and amplexus reflex, being the semen collected after 60 min. The semen volume was 5.76 mL, light-colored. The other characteristics of the semen were: vigor of 4.80, motility of 93%, concentration of 14.24 × 106 mL-1, and content of normal... |
| Tipo: Reprodução |
Palavras-chave: 5.06.03.00-0; 5.05.04.00-2; 2.04.06.00-2 reproduction; Semen; Spermatozoid; Sperm motility Aquicultura. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14780 |
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