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Oliveira,Sarah Ragonha de; Souza,Rondon Tatsuta Yamane Baptista de; Brasil,Elenice Martins; Andrade,Jaqueline Inês Alves de; Nunes,Érica da Silva Santiago; Ono,Eduardo Akifumi; Affonso,Elizabeth Gusmão. |
In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas hydrophila; 96-h LD50; Matrinxã; Tropical fishes. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019 |
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