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| Lazar, Cassandre Sara. |
| A large portion of the greenhouse gas methane on Earth is trapped in marine sediments. However, little methane actually reaches the hydrsphere and the atmosphere, because it is efficiently consumed by anaerobic methane-oxidizing Archaea (ANME). This methane is mainly biogenic in marine sédiments, and is produced by methanogenic Archaea. Methane ascends from deep sources to the seabed, in cold seep sites of continental margins. In order to study microbial community diversity in cold seeps, and to determine geochemical factors that control these microbial communities, we compared four geochemically and geologically distinct sites. In this work, molecular tools (PCR, RT-PCR, DGGE, cloning) as well as genetic markers (16S rRNA, mcrA, dsrB) were employed, to... |
| Tipo: Text |
Palavras-chave: Diversité moléculaire; Archaea; Méthanogène; ANME; McrA; DsrB; DGGE; Volcan de boue; Pockmark; Saumures; Fluides froids; Méthane; Mer de Norvège; Mer Méditerranée; Sédiments.; Molecular diversity; Archaea; Methanogen; ANME; McrA; DsrB; DGGE; Mud volcano; Pockmark; Brines; Cold seep; Methane; Norwegian Sea; Mediterranean Sea; Sediments.. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00031/14250/11534.pdf |
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| Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne. |
| The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers... |
| Tipo: Text |
Palavras-chave: Microbial communities; Pyrosequencing; MBG-D; ANME; Faunal assemblages; Vesicomyidae; Siboglinidae; Microbial mat. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00288/39889/38434.pdf |
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| Carrier, Vincent; Svenning, Mette M.; Grundger, Friederike; Niemann, Helge; Dessandier, Pierre-antoine; Panieri, Giuliana; Kalenitchenko, Dimitri. |
| Cold seeps are characterized by high biomass, which is supported by the microbial oxidation of the available methane by capable microorganisms. The carbon is subsequently transferred to higher trophic levels. South of Svalbard, five geological mounds shaped by the formation of methane gas hydrates, have been recently located. Methane gas seeping activity has been observed on four of them, and flares were primarily concentrated at their summits. At three of these mounds, and along a distance gradient from their summit to their outskirt, we investigated the eukaryotic and prokaryotic biodiversity linked to 16S and 18S rDNA. Here we show that local methane seepage and other environmental conditions did affect the microbial community structure and composition.... |
| Tipo: Text |
Palavras-chave: Arctic; Methane seeps; Prokaryotes; Methanotrophs; ANME; Sulfate-reducing bacteria; Eukaryotes; Foraminifera. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00667/77893/80077.pdf |
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