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| Botto,Adrian E. Cambindo; Muñoz,Juan C.; Giono,Luciana E.; Nieto-Moreno,Nicolás; Cuenca,Carmen; Kornblihtt,Alberto R.; Muñoz,Manuel J.. |
| Abstract Splicing, the process that catalyzes intron removal and flanking exon ligation, can occur in different ways (alternative splicing) in immature RNAs transcribed from a single gene. In order to adapt to a particular context, cells modulate not only the quantity but also the quality (alternative isoforms) of their transcriptome. Since 95% of the human coding genome is subjected to alternative splicing regulation, it is expected that many cellular pathways are modulated by alternative splicing, as is the case for the DNA damage response. Moreover, recent evidence demonstrates that upon a genotoxic insult, classical DNA damage response kinases such as ATM, ATR and DNA-PK orchestrate the gene expression response therefore modulating alternative splicing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA damage; Alternative splicing; ATR; ATM; DNA-PK. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200310 |
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| Marcelain,Katherine; de la Torre,Consuelo; González,Patricio; Pincheira,Juana. |
| Checkpoint response to DNA damage involves the activation of DNA repair and G2 lengthening subpathways. The roles of nibrin (NBS1) and the ATM/ATR kinases in the G2 DNA damage checkpoint, evoked by endogenous and radio-induced DNA damage, were analyzed in control, A-T and NBS lymphoblast cell lines. Short-term responses to G2 treatments were evaluated by recording changes in the yield of chromosomal aberrations in the ensuing mitosis, due to G2 checkpoint adaptation, and also in the duration of G2 itself. The role of ATM/ATR in the G2 checkpoint pathway repairing chromosomal aberrations was unveiled by caffeine inhibition of both kinases in G2. In the control cell lines, nibrin and ATM cooperated to provide optimum G2 repair for endogenous DNA damage. In... |
| Tipo: Journal article |
Palavras-chave: Ataxia-telangiectasia; ATM; ATR kinases; Caffeine; G2 checkpoint; Nibrin (Nbs1); Nijmegen breakage syndrome (NBS). |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200007 |
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