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Registros recuperados: 33
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Ex-vitro establishment of Phalaenopsis amabilis seedlings in different substrates - doi: 10.4025/actasciagron.v33i3.3950 Agronomy
Venturieri, Giorgini Augusto; Universidade Federal de Santa Catarina; Arbieto, Elsa Aurora Mendoza de; Estagiária voluntária do Lab. de Evolução Aplicada UFSC/CCB-BEG.
Eight substrates were evaluated for ex-vitro establishment of Phalaenopsis amabilis seedlings. They were: Fibraflor® (commercial substrate), Turfa Fértil FG2® (commercial substrate), vermiculite, coconut fiber from the pericarp, decomposed pine bark, organic compost, sphagnum and shredded xaxim, in the establishment of seedlings of Phalaenopsis amabilis L., in combination, with and without immersion of the seedlings, after they were removed from the growth flasks, in Manzate 800 at a dosage of 1 g L1. The evaluated parameters were: the sum of the lengths of the two largest leaves, the sum of the lengths of the three largest roots and the percentage of surviving seedlings, evaluated at 190 days after planting. It was observed that there were...
Palavras-chave: 5.00.00.00-4 5.01.03.04-0 5.01.04.01-2 Mancozeb; Acclimatization; Ex-vitro; Damping off Ciências Agrárias Produção de Mudas Floricultura Mancozeb; Acclimatization; Ex-vitro; Damping off.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3950
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Aclimatização de Cattleya (Orchidaceae), em substratos alternativos ao xaxim e ao esfagno - DOI: 10.4025/actasciagron.v30i4.5299 Agronomy
Lone, Alessandro Borini; UEL; Barbosa, Cristiane Muniz; UEL; Takahashi, Lúcia Sadayo Assari; UEL; Faria, Ricardo Tadeu de; UEL.
O xaxim desfibrado e o esfagno são os substratos mais utilizados pelos produtores brasileiros para aclimatização de orquídeas, porém ambos correm o risco de serem extintos e a coleta está proibida pelo Ibama. Desta forma, o objetivo do trabalho foi avaliar a eficiência de substratos alternativos no desenvolvimento de plântulas de Cattleya intermédia, durante a etapa de aclimatização. Os substratos testados foram: xaxim desfibrado; esfagno; casca de arroz carbonizada; casca de pínus + fibra de coco (1:1 v v-1); casca de pínus; fibra de coco. Após dez meses, foram avaliados: comprimento de parte aérea; comprimento da maior raiz; número de pseudobulbos; número de raízes; massa fresca total; pH do substrato; condutividade elétrica do substrato. Verificou-se...
Palavras-chave: 5.01.00.00-9 Agronomia Cattleya; Substratos; Aclimatização. Cattleya; Substrates; Acclimatization.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5299
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Crescimento de mudas micropropagadas da bananeira cv. Nanicão, em diferentes substratos e fontes de fertilizante - DOI: 10.4025/actasciagron.v30i3.3545 Agronomy
Nomura, Edson Shigueaki; APTA-Pólo Vale do Ribeira; Lima, Juliana Domingues; UNESP; Garcia, Valéria Augusta; APTA; Rodrigues, Domingues Sávio; APTA.
Com objetivo de avaliar o efeito de diferentes substratos combinados a fontes de fertilizantes no crescimento de mudas de bananeira micropropagadas cv. Nanicão (Musa spp. AAA), foi instalado um experimento em blocos casualizados, no esquema fatorial 5 x 3, com quatro repetições. Os substratos utilizados foram: S1 - Terra de subsolo + casca de arroz carbonizada + Rendmax Floreira®; S2 - Terra de subsolo + casca de arroz carbonizada + Organifol®; S3 - Terra de subsolo + casca de arroz carbonizada + Organifol® 9% SiO; S4 - Technes Vivatto®; S5 - Areia grossa + casca de arroz carbonizada + Rendmax Floreira®. As fontes de fertilizantes foram: SA - sem adubo; LL - adubo de liberação lenta - Osmocote® 3M 14-14-14 (5,0 kg m-3) misturado ao substrato; e LN - adubo...
Palavras-chave: 5.01.00.00-9 Agronomia Musa ssp.; Aclimatação; Produção de mudas 5.01.03.00-8 Fitotecnia Musa spp.; Acclimatization; Seedling production.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3545
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In vitro propagation of lemon verbena: a plant native of South America Biological Sciences
Pinheiro, Marcos Vinícius Marques; Fontana, Daniele Cristina; Santos, Jullie dos; Pretto, Matheus Milani; Azevedo, Gabrieli Cristina Vitalli de; Schmidt, Denise.
In vitro propagation increases the supply and commercialisation of products of interest. For this, optimising the growing conditions and the composition of the culture medium is crucial to benefit the full development of the plants. Thus, the objective was to evaluate the in vitro propagation of Aloysia triphylla on different culture media, with varying agar and sucrose concentrations. The experiment was conducted as a completely randomised design, 3×3×3 factorial scheme, with three culture media (MS, JADS and WPM), three sucrose concentrations (8, 10 and 12 g L-1) and three agar concentrations (15, 30 and 45 g L-1), with three replicates each and experimental units composed of one plant per replicate. After 25 days of cultivation, the fresh and dry mass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aloysia triphylla; Morphological disorders; Hyperhidricity; Acclimatization.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47105
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In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio) Biol. Res.
LÓPEZ,MAURICIO; FIGUEROA,JAIME; KAUSEL,GUDRUN; VERA,MARÍA INÉS; KRAUSKOPF,MANUEL.
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the...
Tipo: Journal article Palavras-chave: Acclimatization; In situ hybridization; Somatolactin expression; Pituitary; Carp; Fish.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010
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Acclimatization to chronic intermittent hypoxia in mine workers: a challenge to mountain medicine in Chile Biol. Res.
Farias,Jorge G; Jimenez,Daniel; Osorio,Jorge; Zepeda,Andrea B; Figueroa,Carolina A; Pulgar,Victor M.
In the past two decades, Chile has developed intense mining activity in the Andes mountain range, whose altitude is over 4,000 meters above sea level. It is estimated that a workforce population of over 55,000 is exposed to high altitude hypobaric hypoxia. The miners work under shift systems which vary from 4 to 20 days at the worksite followed by rest days at sea level, in a cycle repeated for several years. This Chronic Intermittent Hypoxia (CIH) constitutes an unusual condition for workers involving a series of changes at the physiological, cellular and molecular levels attempting to compensate for the decrease in the environmental partial pressure of oxygen (PO2). The mine worker must become acclimatized to CIH, and consequently undergoes an acute...
Tipo: Journal article Palavras-chave: High Altitude; Mine Workers; Intermittent Hypoxia; Acclimatization; Chilean Model.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100009
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Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl BABT
Siddique,Iram; Bukhari,Najat Abdullah Wahab; Perveen,Kahkashan; Siddiqui,Iffat.
ABSTRACTAn effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 µM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokinins tested, TDZ (5.0 µM) was optimum in inducing multiple shoots as compared to BA and Kin. The rate of shoot multiplication was increased when optimal concentration (5.0 µM) of BA and Kin was tested with different concentration (0.1-1.0 µM) of Indole-3- acetic acid (IAA). Among all the combinations tested, the maximum rate of shoot multiplication was obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acclimatization; Growth regulator; Indole-3- butyric acid; Shoot tip; Senna.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500686
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Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil BABT
Silveira,Daniela Garcia; Souza,Fernanda Vidigal Duarte; Pelacani,Claudinéia Regina; Souza,Antonio da Silva; Ledo,Carlos Alberto da Silva; Santana,José Raniere Ferreira de.
Neoglaziovia variegata (Arr. Cam.) Mez is a Bromeliaceae native to the Caatinga, used for fiber extraction in the Northeast Region of Brazil. The antropic activity has place this species among the threatened ones. The objective of the work was to establish an in vitro propagation and conservation of caroá. Seeds were cultivated in MS medium in the presence or absence of light. In vitro germinated seedlings were multiplied in MS medium supplemented with the combinations 0.05 and 0.50 µM NAA and 2.2 and 4.4 µM BAP and KIN. The best percentages of germination were obtained with the seeds incubated in the presence of light. The highest multiplication ratio was obtained for the NAA (0,5 µM) + BAP (4,4 µM) treatment and the number of roots, with NAA (0.5 µM) +...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acclimatization; Bromeliaceae; Fiber extraction; In vitro preservation; Seedling production.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400016
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Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L. BABT
Bajpai,Anju; Kalim,Shahina; Chandra,Ramesh; Kamle,Madhu.
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supplemented with 2.0 mgL-1 2, 4-D, 400 mgL-1 L-glutamine, 6% sucrose and 500 mgL-1 Malt extract. Following SE induction different developmental stages of somatic embryos (Globular, heart-shaped, torpedo, cotyledonary) was directly obtained and further recurrent embryogenesis also obtained upon prolonged incubation in induction media. Addition of polyethylene glycol (50...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic embryogenesis; Zygotic embryo; Recurrent; Maturation; Acclimatization; Induction; Regeneration.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100412
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Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets Braz. J. Plant Physiol.
Stancato,Giulio Cesare; Tucci,Maria Luiza Sant’Anna.
Estimulation of autotrophy in in vitro plantlets could be achieved through changes in the culture medium, or by changing the traditional hermetic caps by one that could allow gas exchanges between the culture and the environment. Besides that, the use of lamps with distinct emission spectrum irradiaction has propitiated successful results. This work was carried out aiming to evaluate the either the combined or the single action of some factors that can induce autotrophy on in vitro A. andraeanum cv. Eidibel plantlets. 3 sucrose concentrations were used: 0, 15 and 60 mM and for each one, to kinds of flasks according to the cap ventilation: under (0.038 L.h-1) and without ventilation. Flasks were kept under cold light fluorescent lamps or under gro-lux...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acclimatization; Araceae; Plantlets metabolism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100007
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Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds Braz. J. Plant Physiol.
Luis,Zanderluce G.; Bezerra,Kadja Milena G.; Scherwinski-Pereira,Jonny Everson.
Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elaeis guineensis; Arecaceae; Germination; Embryo culture; Acclimatization; Plantlets metabolism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300008
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Influence of Vessel Type, Physical State of Medium and Temporary Immersion on the Micropropagation of Three Rhodophiala species Chilean J. Agric. Res.
Muñoz,Manuel; Seemann,Peter; Jara,Gloria; Riegel,Ricardo.
Rhodophiala C. Presl (Amaryllidaceae) is a genus of attractive flowering geophytes native to South America. They have ornamental value, but most species are little known and have conservation problems. The objective of this study was to optimize a micropropagation process to support the use and preservation of three Chilean native species, R. montana (Phil.) Traub, R. splendens (Rengifo) Traub., and R. ananuca (Phil.) Traub. The research evaluated the feasibility of implementing liquid medium culture and assessed the influence of different tissue culture systems on the shoot production and biomass increment of small bulbs. Three experiments were carried out. The first one determined the influences of flask size and volume of media; the second compared...
Tipo: Journal article Palavras-chave: TIS; Liquid culture; Micropropagation; Acclimatization.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400014
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Adventitious shoots from nodule cluster cultures of Vriesea reitzii: an endemic and endangered bromeliad from atlantic forest Ciência Rural
Rech Filho,Arlindo; Vesco,Lírio Luiz Dal; Guerra,Miguel Pedro.
The Atlantic Forest is a biome with megadiversity and a number of bromeliads take part of it. This is the case of Vriesea reitzii, an endemic bromeliad threatened with extinction. Tissue culture techniques are valuable tools for the mass propagation of bromeliads, thus reducing pressure in the natural habitat. The aim of the present work was to establish an in vitro protocol based on the induction and proliferation of nodule cluster cultures of this species. Plantlets maintained in MS liquid culture medium plus NAA (2µM) and BAP (4µM) had the basal regions of leaves excised and then inoculated in gelled with agar (7g L-1) MS culture medium plus with Dicamba (2.5; 5; 10; 20 e 30µM) and Kin (2µM) or free of plant growth regulators. Nodule cluster cultures...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliads; Microshoots; In vitro regeneration; Conservation; Acclimatization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000300043
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Micropropagation of Chapada Diamantina ornamental bromeliad Ciência Rural
Lima,Andressa Priscila Piancó Santos; Brito,Alone Lima; Santana,José Raniere Ferreira de.
ABSTRACT: Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30-...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro propagation; Acclimatization; Sincoraea mucugensis; NAA.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200400
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Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae) Ciência Rural
Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo.
ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203
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Cultivo in vitro de Stevia rebaudiana Bertoni. Colegio de Postgraduados
Vázquez Baxcajay, Liberia.
Stevia es un género de plantas fanerógamas perteneciente a la familia de las asteráceas, tiene 407 especies; una de sus especies es Stevia rebaudiana Bertoni, nombrada así por Moisés de Santiago Bertoni. Para el establecimiento in vitro de Stevia rebaudiana se aplicaron cinco tratamientos de desinfestación y se obtuvo al usar Cloro® (1.8 % cloro activo), Ridomil y Fungimycin previo a la siembra in vitro y al agregar al medio Cefotaxima (500 mgL-1) se obtuvo un porcentaje de contaminación de 50 % permitiendo una supervivencia de 47 %. En la inducción de callo se mostro que al combinar 1.5 mgL-1 2,4-D/AIA la capacidad de formación de callo del explante de hoja es de 25 %. El tratamiento de cinetina (1.3 gL-1) en el proceso micropropagación de Stevia mostro...
Palavras-chave: In vitro; Micropropagación; Aclimatación; Stevia; Micropropagation; Acclimatization; Maestría; Fisiología Vegetal.
Ano: 2012 URL: http://hdl.handle.net/10521/751
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Aclimatización de plantas de paulownia (Paulownia tomentosa) en 13 sustratos Colegio de Postgraduados
Sánchez Bolón, Judith.
La aclimatización es el periodo donde las plantas propagadas in vitro tienen cambios de ambiente, tipo de sustrato, entre otros factores controlados por el hombre, que determinan la sobrevivencia. Se evaluaron mezclas de materiales orgánicos (composta de cachaza y bagazo de caña de azúcar, suelo y turba) e inorgánicos (tezontle y agrolita) para determinar el porcentaje de sobrevivencia en la fase de aclimatización y crecimiento de plantas de Paulownia tomentosa propagadas in vitro. Las variables evaluadas fueron el porcentaje de sobrevivencia, altura de planta y formación de hojas, área foliar, longitud de raíz y tallo, relación raíz parte aérea, morfología de la raíz, peso de biomasa fresca y seca, concentración nutrimental del tejido vegetal y...
Tipo: Tesis Palavras-chave: Paulownia tomentosa; Aclimatización; Propagación in vitro; Sustratos; Maestría; Edafología; Acclimatization; Propagation in vitro; Substrates.
Ano: 2008 URL: http://hdl.handle.net/10521/1238
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In vitro propagation of five Alocasia species Horticultura Brasileira
Bhatt,Arvind; Stanly,Christine; Keng,Chan Lai.
The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alocasia amazonica; A. cuprea; A. robusta; A. longiloba; A. chaii; Culture medium; Acclimatization.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006
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Micropropagation of Homalomena pineodora Sulaiman & Boyce (Araceae): a new species from Malaysia Horticultura Brasileira
Stanly,Christine; Bhatt,Arvind; Sulaiman,Baharuddin; Keng,Chan Lai.
Homalomena pineodora (family Araceae) is a species found to have impressive foliage characteristics which remain evergreen throughout the year. Therefore, H. pineodora can be grown as an ornamental plant. Generally H. pineodora needs 3-5 years to propagate and multiply. However, the demand for new ornamental plants is increasing worldwide and the quality of planting material is a basic need for boosting productivity. Therefore an efficient micropropagation protocol for large-scale production of H. pineodora was developed. In vitro shoot cultures were initiated from the rhizomatous buds on MS basal medium. The best conditions for propagating H. pineodora was found to be MS medium supplemented with 3% sucrose and 0.5 mg L-1 BA (6-benzyladenine) under 24 h of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Homalomena pineodora; Acclimatization; Scanning electron microscopy; Cytokinin; In vitro culture; Shoot proliferation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000100007
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Адаптация Spirulina platensis к изменениям культуральной среды IBSS Repository
Харчук, И. А.; Алисиевич, А. В..
Измерены морфометрические характеристики Spirulina platensis в зависимости от концентрации питательной среды. Показано, что изменение концентрации питательной среды вызывает распад трихомов на гормогонии. Длина молодых гормогониев равна 0–300 мк, доминируют фрагменты до 100 мк (80-90 %). Размеры основного количества гормогониев составляют 20–40 мк. Установлено, что дробление трихом случайно, закономерности в расположении некроидальных клеток («жертвенных») не выявлено. http://biotex.ibss.org.ua/
Tipo: Journal Contribution Palavras-chave: Трихом; Гормогоний; Некроидные клетки; Адаптация; Spirulina platensis; Trichom; Hormogones; Necrosis cells; Acclimatization.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1749
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