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Pollen grain viability in accessions of Crotalaria juncea L. (FABACEAE) Agrociencia
Coelho,Ana P. D.; Morais,Katiule P.; Dail Laughinghouse IV,Haywood; Giacomini,Sandro J.; Tedesco,Solange B..
Studies on pollen viability are useful for breeding Crotalaria juncea L. since they provide information that will increase the chance of successful intraspecific crossing. The aim of this study was to evaluate pollen viability in C. juncea accessions cultivated in Rio Grande do Sul, Brazil. Inflorescences of 10 accessions of C. juncea were collected, fixed in absolute ethanol: glacial acetic acid (3:1) for 72 h at room temperature and placed in 70 % ethanol. Analysis of pollen viability was performed with slides of anthers using the squashing technique and staining with acetic orcein 2 % and Alexanders stain. Means were compared using the Tukey test (p≤0.05). Pollen viability was high and significantly different among the accessions; the highest value was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalaria juncea Alexander's stain; Acetic orcein; Pollen viability; Plant cytogenetics.
Ano: 2012 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000500005
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Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods Biocell
Frescura,Viviane Dal-Souto; Laughinghouse IV,Haywood Dail; Dorow,Thais Scotti do Canto-; Tedesco,Solange Bosio.
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Pollen grains; Barba-de-bode; Acetic orcein; Acetic carmine; Alexander's stain.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300007
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