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Influence of medium constituents on the biosynthesis of cephalosporin-C Electron. J. Biotechnol.
Kumar Nigam,Vinod; Verma,Ruchi; Kumar,Abhishek; Kundu,Subir; Ghosh,Purnendu.
Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4% are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC
Tipo: Journal article Palavras-chave: Acremonium chrysogenum; Batch fermentation; Cephalosporin-C; Methionine; Stirred tank bioreactor.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200007
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Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum BABT
Vialta,Airton; Catani,Cleide F; B. Junior,Renato; Azevedo,João L.
Mutants unable to convert exogenous sulfate to sulfite were isolated using the toxic analogue selenate. Three of twenty-eight isolated mutants were chromate sensitive. They showed a possible lesion in the gene that codes the ATP sulfurylase. The others were chromate resistant, and probably had a lesion in one or both of the genes that code the sulfate permease. Methionine increased the resistance levels to selenate. In addition, the frequency of spontaneous mutants obtained in a medium containing methionine was higher (between 2.4 x 10-6 and 18.0 x 10-6) than that obtained using a medium without any intentional source of sulfur (between 0.7 x 10-6 and 5.0 x 10-6). The original strain, as well as the mutants, were able to grow in a sulfur-free liquid medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Selenate resistant mutant; Acremonium chrysogenum; Cephalosporin C.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300016
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