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	Provedor de dados BJMBR (1) International Journal of Morphology (1) Autor Beier,John C (1) Gicheru,Michael M (1) Githure,John I (1) Hu,T. (1) Injera,Wilfred E (1) Jiao,X. (1) Kabiru,Elphantus W (1) Shi,J. (1) Yin,X. (1) Zhou,J. (1) Mais... Palavra-chave Adherent cells (2) Activated hemocytes (1) Annexin V (1) Apoptosis (1) Flow cytometry (1) Immunopathological features (1) Lactadherin (1) Midgut epithelium (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2013 (1) 2008 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Immunopathological Features Developing in the Mosquito Midgut after Feeding on Anopheles gambiae Mucin-1 / Interleukin-12 cDNA Immunized Mice International Journal of Morphology Injera,Wilfred E; Kabiru,Elphantus W; Gicheru,Michael M; Githure,John I; Beier,John C. The mosquito midgut is the organ into which the blood meal passes and in which, within a peritrophic membrane secreted by the epithelium, the blood is retained during digestion and absorption. The mosquito midgut is lined with an actin filled microvilli that are exposed to the harsh environment of the gut lumen such as food particle abrasion, digestive hydrolases and attack by pathogens and parasites that are taken in by the blood meal. These microvilli are protected them these effects by the peritrophic matrix, the glycocalyx and the mucin proteins that line their epithelial surfaces. Immunization of BALB/c mice with AgMUC1/IL-12 cDNA has been shown to kill mosquitoes when fed on these mice. Mucin is one of the proteins produced in the mosquito midgut... Tipo: Journal article Palavras-chave: Midgut epithelium; Immunopathological features; Adherent cells; Activated hemocytes. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100051 Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells BJMBR Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.. Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization... Tipo: Info:eu-repo/semantics/article Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002 Registros recuperados: 2 Primeira ... 1 ... Última

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