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| Zuccari,D.A.P.C; Castro,R; Jardim,B.V; Mancini,U.M; Polachini,G.M. |
| Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Adhesion molecule; Cancer; Western blot; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100005 |
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| Naito,Yuji; Shimozawa,Makoto; Manabe,Hiroki; Kuroda,Masaaki; Tomatsuri,Naoya; Uchiyama,Kazuhiko; Takagi,Tomohisa; Yoshida,Norimasa; Yoshikawa,Toshikazu. |
| Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol.... |
| Tipo: Journal article |
Palavras-chave: Adhesion molecule; Monocyte-endothelial cell interaction; Oxysterol; Red wine extract. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009 |
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