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Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin BABT
Manera,Ana Paula; Kamimura,Eliana Setsuko; Brites,Luciana Machado; Kalil,Susana Juliano.
Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyloglucosidase; Adsorption isotherm; Kinetics adsorption; Ion exchange resin; Purification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
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Study of adsorption isotherms and physicochemical characterization of natural food colourants Ciênc. Tecnol. Aliment.
VIANA,Kéllen Wanessa Coutinho; OLIVEIRA,Isadora Rebouças Nolasco de; ROCHA,Juliana de Cássia Gomes; BARROS,Frederico Augusto Ribeiro de; STRINGHETA,Paulo César.
Abstract Synthetic colourants are widely used for colouring foods. However, their safety has been questioned. Due to this limitation and the global trend of consuming healthy foods, the industry is faced with the need to replace artificial colourings, which has given rise to a growing interest in colourants from natural sources. Thus, this work obtained the moisture adsorption isotherms of the natural food dyes of anthocyanins, betalains and curcumin and evaluated the humidity, water activity (aw), hygroscopicity, colour and antioxidant capacity in vitro. Isotherms were determined by the static gravimetric method with saturated salt solutions (LiCl. H2O, MgCl2.6H 2O, Mg(NO3)2.6H2O, NaCl, KCl and K2 SO4) at 25 °C. Experimental data were adjusted by GAB,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural dyes; Anthocyanins; Betalains; Curcumin; Adsorption isotherm.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000500074
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Equilibrium sorption isotherm studies of Cd(II), Pb(II) and Zn(II) ions detoxification from waste water using unmodified and EDTA-modified maize husk Electron. J. Biotechnol.
Igwe,Jude C; Abia,Augustin A.
The mobilization of heavy metals in the environment due to industrial activities is of serious concern due to the toxicity of these metals in humans and other forms of life. The equilibrium adsorption isotherms of Cd(II), Pb(II) and Zn(II) ions, detoxification from waste water using unmodified and EDTA-modified maize husk have been studied. Maize husk was found to be an excellent adsorbent for the removal of these metal ions. The amount of these metal ions adsorbed increased as the initial concentration increased. Also, EDTA-modification enhanced the adsorption capacity of maize husk due to the chelating ability of ethylenediamine tetra acetic acid (EDTA). Among the three adsorption isotherms tested, Dubinin-Radushkevich isotherm gave the best fit with R²...
Tipo: Journal article Palavras-chave: Adsorption isotherm; EDTA; Heavy metals; Maize husk; Waste water.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400007
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Zinc adsorption in highly weathered soils PAB
Casagrande,José Carlos; Soares,Marcio Roberto; Mouta,Ernesto Rinaldi.
The objective of this work was to assess the effects of pH and ionic strength upon zinc adsorption, in three highly weathered variable charge soils. Adsorption isotherms were elaborated from batch adsorption experiments, with increasing Zn concentrations (0-80 mg L-1), and adsorption envelopes were constructed through soil samples reactions with 0.01, 0.1 and 1 mol L-1 Ca(NO3)2 solutions containing 5 mg L-1 of Zn, with an increasing pH value from 3 to 8. Driving force of reaction was quantified by Gibbs free energy and separation factor. Isotherms were C-, H- and L-type and experimental results were fitted to nonlinear Langmuir model. Maximum adsorption ranged from 59-810 mg kg-1, and Zn affinity was greater in subsoil (0.13-0.81 L kg-1) than in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PH; Adsorption envelope; Adsorption isotherm; Ionic strength; Trace element.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000100017
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