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Registros recuperados: 45
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Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae Acta Amazonica
Pohlit,Adrian Martin; Quinard,Etienne Louis Jacques; Nunomura,Sergio Massayoshi; Tadei,Wanderli Pedro; Hidalgo,Ari de Freitas; Pinto,Ana Cristina da Silva; Santos,Elba Vieira Mustafa dos; Morais,Sabrina Kelly Reis de; Saraiva,Rita De Cássia Guedes; Ming,Lin Chau; Alecrim,Alexandre Mascarenhas; Ferraz,Alfeu de Barros; Pedroso,Andreza Cristiana da Silva; Diniz,Elisangela Vieira; Finney,Ellen Kathryn; Gomes,Erika de Oliveira; Dias,Hercules Bezerra; Souza,Katiuscia dos Santos de; Oliveira,Laura Cristina Pereira de; Don,Luciana de Castro; Queiroz,Maria Mireide Andrade; Henrique,Marycleuma Campos; Santos,Mirian dos; Lacerda Júnior,Orivaldo da Silva; Pinto,Patrícia de Souza; Silva,Suniá Gomes; Graça,Yara Rodrigues.
Ethanol, methanol and water extracts representing mostly native plant species found in the Amazon region were prepared, respectively, by maceration, continuous liquid-solid extraction and infusion, followed by evaporation and freeze-drying. The freeze-dried extracts were tested for lethality toward Aedes aegypti larvae at test concentrations of 500 mg / mL. In general, methanol extracts exhibited the greatest larvicidal activity. The following 7 methanol extracts of (the parts of) the indicated plant species were the most active, resulting in 100% mortality in A. aegypti larvae: Tapura amazonica Poepp. (root), Piper aduncum L. (leaf and root), P. tuberculatum Jacq. (leaf, fruit and branch). and Simaba polyphylla (Cavalcante) W.W. Thomas (branch).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Larvicide; Aedes aegypti; Tapura; Piper; Simaba; Amazonia.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672004000100012
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Larvicidal, cytotoxic and genotoxic effects of aqueous leaf extract of Jatropha mollissima (Pohl) Baill Biological Sciences
Araújo, José Rafael Silva; Costa, Maria Wlly da Silva; Oliveira, Wallace Baldez; Cavalcante, Reginaldo Roris; Almeida, Pedro Marcos; Martins, Francielle Alline.
 The present study aimed to evaluate the larvicidal effect of aqueous leaf extract from Jatropha mollissima on the larvae of Aedes aegypti and analyze its cytotoxic and genotoxic activity in the Allium cepa test. Larvae of the mosquito were exposed to the negative and positive controls (distilled water and diflubenzuron, 0.003 mg mL-1, respectively) and to leaf extract concentrations of 0.001, 0.005, 0.01, 0.02, 0.04, 0.06, 0.08 and 0.1 mg mL-1. The mortality rate was evaluated every 24 hours over five days. For the cytotoxic and genotoxic analyses, roots of A. cepa were exposed to the negative (distilled water) and positive control (trifluralin, 0.84 ppm) and to different leaf extract concentrations (0.01, 0.1, 1 and 10 mg mL-1) for 24 hours. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Allium cepa test; Chromosome alterations; Medicinal plant..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34501
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Larvicidal activity of the water extract of Moringa oleifera seeds against Aedes aegypti and its toxicity upon laboratory animals Anais da ABC (AABC)
Ferreira,Paulo M.P.; Carvalho,Ana F.U.; Farias,Davi F.; Cariolano,Nara G.; Melo,Vânia M.M.; Queiroz,Maria G.R.; Martins,Alice M.C.; Machado-Neto,Joaquim G..
In this work, biological effects of the water extract of Moringa oleifera seeds (WEMOS) were assessed on eggs and 3rd instar larvae of Aedes aegypti and on its toxicity upon laboratory animals (Daphnia magna, mice and rats). Crude WEMOS showed a LC50 value of 1260µg/mL, causing 99.2 ± 2.9% larvae mortality within 24 h at 5200µg/mL, though this larvicidal activity has been lost completely at 80ºC/10 min. WEMOS did not demonstrate capacity to prevent egg hatching. After extensive dialyses of the crude WEMOS into watersoluble dialyzable (DF) and nondyalizable (NDF) fractions, only DF maintained its efficacy to kill larvae. Acute toxicity evaluations on daphnids (EC50 of 188.7µg/mL) and mice (LD50 of 446.5 mg/kg body weight) pointed out to low toxicity....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Daphnia magna; Larvicidal activity; Moringa oleifera seeds; Toxicity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000200007
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Chemical composition and biological activities of the essential oils from Vitex-agnus castus, Ocimum campechianum and Ocimum carnosum Anais da ABC (AABC)
RICARTE,LARA P.; BEZERRA,GABRIELI P.; ROMERO,NIRLA R.; SILVA,HORLANDO C. DA; LEMOS,TELMA L.G.; ARRIAGA,ANGELA M. C.; ALVES,PÉRICLES B.; SANTOS,MARCELO B. DOS; MILITÃO,GARDENIA C.G.; SILVA,THIAGO D.S.; BRAZ-FILHO,RAIMUNDO; SANTIAGO,GILVANDETE M.P..
Abstract The essential oils obtained by hydrodistillation from fresh leaves of Vitex agnus-castus and Ocimum campechianum, and from fresh inflorescences of Ocimum carnosum were analysed by GC-FID and GC-MS. The major components of V. agnus-castus essential oil were identified as 1,8-cineole (47.9%), terpinyl α-acetate (11.6%), sabinene (11.2%) and caryophyllene oxide (9.7%), while in the O. campechianum essential oil were eugenol (72.1%), β-elemene (6.8%), (E)-caryophyllene (6.4%) and bicyclogermacrene (5.2%). Linalool (79.0%), α-epi-cadinol (5.4%), terpinen-4-ol (3.2%) and 1,8-cineole (2.8%) were the major constituents in the O. carnosum essential oil. The essential oils were subsequently evaluated for their larvicidal and cytotoxic activities. Larval...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Cytotoxic activity; Lamiaceae; Larvicidal activity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100718
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Evaluation of embryotoxic and embryostatic effects of the aqueous extract of Rhizophora mangle and tannic acid on eggs and larvae of Aedes aegypti Anais da ABC (AABC)
RODRIGUES NETO,AGEU A.; GOMES JÚNIOR,PLÍNIO P.; SILVA,MAURICIO C.; LIMA,CLÁUDIA S.A.; YARA,RICARDO; GUIMARÃES,EMÍLIA B.; SANTANA,EDUARDA S. DE; SILVA,LUZIA A. DA; LIRA,EDUARDO J.R.V. DE; VIEIRA,JEYMESSON R.C..
ABSTRACT Rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. The aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of R. mangle and synthetic tannic acid on eggs and larvae of Aedes aegypti. A. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/mL of extract and tannic acid for a period of 14 days. Mineral water was used as a negative control. The eggs were observed and counted in a stereomicroscope (1.2x). In all extract concentrations there was stimulation in hatching in relation to the control, but only in concentration of 750 mg/mL it was statistically significant. In tannic acid (250µg/ml)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Embryostatic; Embryotoxic; Rhizophora mangle; Tannic acid.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502141
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Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L. Anais da ABC (AABC)
Farias,Davi F.; Cavalheiro,Mariana G.; Viana,Martônio P.; Queiroz,Vanessa A.; Rocha-Bezerra,Lady C.B.; Vasconcelos,Ilka M.; Morais,Selene M.; Carvalho,Ana F.U..
This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Larvicidal compounds; Leguminous seeds; Water extracts.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300006
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A potent larvicidal agent against Aedes aegypti mosquito from cardanol Anais da ABC (AABC)
PAIVA,DERISVALDO R.; LIMA,DÊNIS P. DE; AVVARI,NAGA P.; ARRUDA,EDUARDO J. DE; CABRINI,ISAIAS; MARQUES,MARIA RITA; SANTOS,EDSON A. DOS; BIAGGIO,FRANCISCO C.; SANGI,DIEGO P.; BEATRIZ,ADILSON.
ABSTRACT Cardanol is a constituent of Cashew Nut Shell Liquid that presents larvicidal activity against Aedes aegypti. The isolation of cardanol is somewhat troublesome, however, in this work we describe an efficient and inexpensive method to obtain it as a pure material. The compound was used as starting material to make chemical transformation leading to saturated cardanol, epoxides and, halohydrins. These derivatives were tested for toxicity against Aedes aegypti larvae. The results showed that iodohydrins are very promising compounds for making commercial products to combat the vector mosquito larvae presenting a LC50 of 0.0023 ppm after 72 h of exposure.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Insecticide; Larvicide; Phenolic lipids; CNSL.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200373
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Molecular docking of Anopheles gambiae and Aedes aegypti glutathione S-transferases epsilon 2 (GSTE2) against usnic acid: an evidence of glutathione conjugation BABT
Maia,Rafael Trindade; Nadvorny,Daniela.
The aim of this study was to develop a theoretical model using Anopheles gambiae GSTE2 structure as template for Aedes aegypti GSTE2 by homology modeling Docking simulations were performed for both the enzymes against usnic acid in neutral and anionic forms. Ramachandran plot revealed that 93.9% of the GSTE2 model residues were located on most favored regions. Model evaluation was made by the ANOLEA and GROMOS analysis. Docking results indicated that the enzymes were able to form glutathione-conjugate with usnic acid in both the forms (anionic and neutral).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anopheles gambiae; Aedes aegypti; Molecular modeling; Insecticide resistance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500689
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Evaluation of toxic effects with transition metal ions, EDTA, SBTI and acrylic polymers on Aedes aegypti (L., 1762) (Diptera: Culicidae) and Artemia salina (artemidae) BABT
Arruda,Eduardo José de; Rossi,Ana Paula Leão; Porto,Karla Rejane de Andrade; Oliveira,Lincoln Carlos Silva de; Arakaki,Andrea Haruko; Scheidt,Gessiel Newton; Soccol,Carlos Ricardo.
This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect control; Aedes aegypti; Artemia salina.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200012
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Isolation and Characterization of Midgut Lectin From Aedes aegypti (L.) (Diptera: Culicidae) BABT
Ayaad,Tahany Hassan; Al-Akeel,Rasha Khalifah; Olayan,Ebtisam.
ABSTRACT The present investigation deals with the isolation and characterization of a lectin from Aedes aegypti (Ae aegypti) female mid gut extract that agglutinates various mammalian red blood cells (RBCs) such as human three groups A, B, and O (RH+), mouse, rat, guinea-pig, sheep and goat erythrocytes. The highest activity of both crude and isolated mid gut lectins were detected against sheep RBCs. Using (NH4)2 SO4 fractionation, ion-exchange and mannose-CNBr-Sepharose 6B affinity chromatography techniques, Ae. aegypti midgut lectin (Aelec) was purified to homogeneity.Isoelectric focusing (IEF) and reducing SDS/PAGE revealed that the isolated mid gut lectin had isoelectric point (PI) of 5.90, and subunits approximate molecular weights of 35.50 and 27.35...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lectin; Purification; Characterization; Hemagglutination; Aedes aegypti; Mosquitoes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600905
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Differences in the replicative capacities of clinical isolates of dengue virus in C6/36 cells and in urban populations of Aedes aegypti from Colombia, South America BJID
Quintero-Gil,Diana Carolina; Uribe-Yepes,Alexander; Ospina,Marta; Díaz,Francisco Javier; Martinez-Gutierrez,Marlen.
ABSTRACT Dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (DENV) serotypes that co-circulate constantly in hyperendemic areas such as Medellin (Colombia), and these serotypes are transmitted by mosquitoes of the genus Aedes. In this study, we evaluated the replicative capacity of strains isolated in Medellin between 2003 and 2007 in C6/36 cells and in colonies of Aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city. The phylogenetic analysis grouped isolates according to the predominant genotypes found in the Americas, and the in vitro characterization showed differences in the morphological changes induced by the isolates of each of the isolated serotypes compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue; Viral replication; Vector competence; Aedes aegypti; C6/36 cells; Colombia.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400257
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São Paulo urban heat islands have a higher incidence of dengue than other urban areas BJID
Araujo,Ricardo Vieira; Albertini,Marcos Roberto; Costa-da-Silva,André Luis; Suesdek,Lincoln; Franceschi,Nathália Cristina Soares; Bastos,Nancy Marçal; Katz,Gizelda; Cardoso,Vivian Ailt; Castro,Bronislawa Ciotek; Capurro,Margareth Lara; Allegro,Vera Lúcia Anacleto Cardoso.
Urban heat islands are characterized by high land surface temperature, low humidity, and poor vegetation, and considered to favor the transmission of the mosquito-borne dengue fever that is transmitted by the Aedes aegypti mosquito. We analyzed the recorded dengue incidence in Sao Paulo city, Brazil, in 2010-2011, in terms of multiple environmental and socioeconomic variables. Geographical information systems, thermal remote sensing images, and census data were used to classify city areas according to land surface temper- ature, vegetation cover, population density, socioeconomic status, and housing standards. Of the 7415 dengue cases, a majority (93.1%) mapped to areas with land surface temperature >28 ◦ C. The dengue incidence rate (cases per 100,000...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urban heat islands; Land surface temperature; Vegetation cover; Dengue; Aedes aegypti.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200146
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Identification of Zika virus in immature phases of Aedes aegypti and Aedes albopictus: a surveillance strategy for outbreak anticipation BJMBR
Maniero,V.C.; Rangel,P.S.C.; Coelho,L.M.C.; Silva,C.S.B.; Aguiar,R.S.; Lamas,C.C.; Cardozo,S.V..
A progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. The main objective of this article was to identify the presence of Zika (ZIKV), dengue (DENV), and Chikungunya (CHIKV) viruses in immature stages of Aedes aegypti and Ae. albopictus. Household collections of immature phases of the vectors were carried out in the years 2015 and 2016. A total of 2902 dwellings were visited and the rate of infestation with larvae and pupae of Aedes mosquitoes was 283/1462 (19.4%) in March 2015 and 55/1440 (3.8%) in June 2015. In March 2015, 907 larvae/pupae were collected (583 or 64.3% of Ae. aegypti and 324 or 35.7% of Ae. albopictus) while in June 2015 there was a reduction in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zika virus; Aedes aegypti; Aedes albopictus; Arbovirus; Georeferencing; Xenomonitoring.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100605
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Alcaloides de pemoche (Erythrina herbacea L.) como larvicidas contra el mosquito de la fiebre amarilla (Aedes aegypti L.). Colegio de Postgraduados
Salazar Aguilar, Sandra.
El mosquito transmisor de la fiebre amarilla, A. aegypti, también es vector del dengue, enfermedad con importancia a nivel mundial por la problemática socioeconómica provocada en las epidemias. En México los estados considerados económicamente importantes, son áreas potenciales la transmisión de esta enfermedad. Por lo que la búsqueda de sustancias para el control de este organismo es importante, ya que comienza a volverse resistente a insecticidas organosintéticos. Así en el presente estudio se realizaron pruebas con extractos de pemoche Erythrina herbacea, para encontrar posibles sustancias que puedan utilizarse para este fin. Se obtuvieron de las semillas de esta especie, los extractos crudos metanólicos: fracción de alcaloides libres (FAL) y fracción...
Palavras-chave: Erythrina herbacea; Aedes aegypti; Toxicidad de alcaloides; Actividad larvicida; Alcaloide diénico; Toxicity of alkaloids; Larvicida activity; Dienic alkaloid; Maestría; Botánica.
Ano: 2011 URL: http://hdl.handle.net/10521/406
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Alcaloides de pemoche (Erythrina herbacea L.) como larvicidas contra el mosquito de la fiebre amarilla (Aedes aegypti L.). Colegio de Postgraduados
Salazar Aguilar, Sandra.
El mosquito transmisor de la fiebre amarilla, A. aegypti, también es vector del dengue, enfermedad con importancia a nivel mundial por la problemática socioeconómica provocada en las epidemias. En México los estados considerados económicamente importantes, son áreas potenciales la transmisión de esta enfermedad. Por lo que la búsqueda de sustancias para el control de este organismo es importante, ya que comienza a volverse resistente a insecticidas organosintéticos. Así en el presente estudio se realizaron pruebas con extractos de pemoche Erythrina herbacea, para encontrar posibles sustancias que puedan utilizarse para este fin. Se obtuvieron de las semillas de esta especie, los extractos crudos metanólicos: fracción de alcaloides libres (FAL) y fracción...
Palavras-chave: Erythrina herbacea; Aedes aegypti; Toxicidad de alcaloides; Actividad larvicida; Alcaloide diénico; Toxicity of alkaloids; Larvicida activity; Dienic alkaloid; Maestría; Botánica.
Ano: 2011 URL: http://hdl.handle.net/10521/406
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Manejo de la resistencia a insecticidas en el mosquito Aades aegypti (Linneus, 1762) en Guerrero, México. Colegio de Postgraduados
Chino Cantor, Araceli.
El dengue es una de las enfermedades más graves a nivel mundial en salud pública, y a la fecha el uso de insecticidas químicos ha sido la principal medida de control del vector Aedes aegypti transmisor del virus causal del dengue. Con la finalidad de aportar bases para definir una estrategia de manejo regional de insecticidas, se determinó la susceptibilidad de poblaciones de Aedes aegypti L., provenientes de tres regiones de Guerrero, México (Tierra Caliente, Costa Chica y Acapulco), a los insecticidas malatión, temefos, clorpirifós étilico, pirimifós-metil, permetrina, lambda cialotrina, propoxur y espinosina. La población susceptible New Orleans se usó como referencia. Los productos en grado técnico y comercial se evaluaron por aplicación residual en...
Palavras-chave: Dengue; Aedes aegypti; Resistencia a insecticidas; Insecticides resistance; Entomología y Acarología; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/2160
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Selección de hábitat de oviposición en Aedes aegypti (Diptera: Culicidae) mediante estímulos físicos Ecología austral
Badano,Ernesto I; Regidor,Héctor A.
Aedes aegypti puede desarrollar su fase acuática en recipientes artificiales que detecta y evalúa mediante variables físicas. En este estudio se analizó el efecto del color (negro, rojo, verde y blanco) y de la superficie del recipiente (177 cm² y 57 cm²) sobre la respuesta de oviposición de Aedes aegypti en dos ambientes lumínicos diferentes (luz solar y 90% de sombra). Se montaron dos experimentos factoriales, uno en cada situación de luz, combinando colores y superficies. En ambas situaciones de luz se detectaron diferencias entre colores. Al sol, las trampas negras y rojas difirieron de las blancas y verdes; a la sombra, las trampas negras y rojas difirieron solamente de las verdes. Las superficies solo difirieron en la situación de sombra, con una...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Culicidae; Selección de hábitat; Respuesta de oviposición; Argentina.
Ano: 2002 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1667-782X2002000200006
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Desempenho de um pulverizador pneumático costal motorizado sob diferentes condições REA
Gadanha Júnior,Casimiro D.; Costa,Marco A. F. da; Milan,Marcos.
O objetivo do trabalho foi avaliar a uniformidade de vazão de um pulverizador pneumático costal motorizado, utilizado no controle do mosquito vetor da dengue, sob várias condições operacionais de aplicação. O experimento foi planejado como um delineamento inteiramente casualizado, no esquema fatorial 2 x 7, com cinco repetições, sendo os fatores de variação dois diâmetros de orifício do disco dosador e sete ângulos de aplicação em relação ao plano horizontal. Foi avaliado também o efeito das pressões sobre a vazão por meio dos testes de Pearson. A interação dos fatores diâmetro do orifício do disco dosador e ângulo de aplicação foi significativa para a vazão. O disco dosador com diâmetro de 0,42 mm apresentou maior uniformidade entre as vazões médias,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Controle químico; Pulverizador.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162004000300023
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First record of Aedes aegypti (L.) (Diptera: Culicidae) infected by the parasite Ascogregarina culicis (Ross) (Apicomplexa: Lecudinidae) in Argentina Entomología
Dellapé,Mariana E.; Marti,Gerardo A.; Tranchida,María C.; García,Juan J..
In a survey for parasites and pathogens of Aedes aegypti conducted in La Plata, Argentina, an aseptate gregarine was found parasitizing larvae, pupae and adults of this mosquito species. This gregarine species was identified as Ascogregarina culicis based on the morphology and size of gamonts, gametocysts and oocysts. It is the first record of gregarine infections in culicids from Argentina.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Gregarine; Ascogregarina culicis; Aedes aegypti.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0328-03812005000100009
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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies Genet. Mol. Biol.
Paiva,Marcelo Henrique Santos; Guedes,Duschinka Ribeiro Duarte; Leal,Walter Soares; Ayres,Constância Flávia Junqueira.
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
Tipo: Info:eu-repo/semantics/article Palavras-chave: ZIKV; RT-PCR; Aedes aegypti; Vector competence.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597
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Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

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