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| Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming. |
| The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079 |
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| Qin,Peng; Lin,Yu; Hu,Yaodong; Liu,Kun; Mao,Shuangshuang; Li,Zhanyi; Wang,Jirui; Liu,Yaxi; Wei,Yuming; Zheng,Youliang. |
| Abstract The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A. tauschii accessions grown under normal and polyethylene glycol-simulated drought stress conditions and performed a genome-wide association study using 7,185 single nucleotide polymorphism (SNP) markers. We identified 208 and 28 SNPs associated with all traits using the general linear model and mixed linear model, respectively, while both models detected 25 significant SNPs with genome-wide distribution. Public database searches... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aegilops tauschii; Drought resistance; Genome-wide association study; Single nucleotide polymorphism; Wheat. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300398 |
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