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| Parodi,Thaylise Vey; Vargas,Agueda Palmira de Castagna; Krewer,Carina; Flores,Érico Marlon de Moraes; Baldisserotto,Bernardo; Heinzmann,Berta Maria; Oliveira,José Vladimir de; Popiolski,Ariana Secco; Minozzo,Mariane. |
| This study investigated the chemical composition of five different extracts of Aloysia triphylla and their activity against Aeromonas sp. The extracts were obtained from the dried leaves by pressurized CO2 extraction at 30, 50 and 70ºC, and 100, 150, and 200 bar, and analyzed by GC/FID and GC-MS. The antibacterial activity was assayed by the microdilution method. The tested microorganisms comprised seven Aeromonas isolates obtained from the kidney of infected silver catfish, Rhamdia quelen. The yield, chemical composition and antibacterial activity of the extracts were dependent on the extraction conditions. Mono and sesquiterpenoids were the major constituents of all the extracts and the highest extraction yield was obtained at 70ºC and 200 bar. A.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aloysia triphylla; Pressurized carbon dioxide extraction; Aeromonas sp. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200014 |
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| Oh,Chulhong; Nikapitiya,Chamilani; Lee,Youngdeuk; Whang,Ilson; Kang,Do-Hyung; Heo,Soo-Jin; Choi,Young-Ung; Lee,Jehee. |
| An agar-degrading Pseudoalteromonas sp. AG52 bacterial strain was identified from the red seaweed Gelidium amansii collected from Jeju Island, Korea. A β-agarase gene which has 96.8% nucleotide identity to Aeromonas β-agarase was cloned from this strain, and was designated as agaA. The coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (GHF)-16. The predicted molecular mass of the mature protein was 32 kDa. The recombinant β-agarase (rAgaA) was overexpressed in Escherichia coli and purified as a fusion protein. The optimal temperature and pH for activity were 55 ºC and 5.5, respectively. The enzyme had a specific activity of 105.1 and 79.5 unit/mg toward agar and agarose, respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agar; Aeromonas sp; Β-agarase; Pseudoalteromonas sp; GHF-16; Neoagaro-oligosaccharides. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400006 |
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| Rall,Vera Lúcia Mores; Iaria,Sebastião Timo; Heidtmann,Sandra; Pimenta,Fabiana Cristina; Gamba,Rosa Carvalho; Pedroso,Débora Midori Myaki. |
| Aeromonas has been described as an emergent foodborne pathogen of increasing importance. In this study, we report that 48% of 50 Pintado fish samples collected at the retail market of São Paulo city were positive for Aeromonas sp, as detected by the direct plating method. When the presence/absence method was used, the positivity was 42%. A. caviae was the most frequent species, followed by A. hydrophila and A. sobria. Production of cytotoxic enterotoxin, observed in suckling mouse assay, was detected in 67% of A. sobria strains, in 60% of A. hydrophila strains and in 40% of A. caviae strains. In vitro tests, performed with HEp-2 cells, showed that 88% of A. hydrophila, 27% of A. sobria and 13% of A. caviae strains were positive for this toxin. The in vivo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas sp; Virulence factors; Drug susceptibility; Fish. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300015 |
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