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	Provedor de dados BABT (1) BJMBR (1) Autor Almeida,Lia Magalhães de (1) Batista-Viera,F. (1) Braga,Renata Chastinet (1) Carlsson,J. (1) Franco-Fraguas,L. (1) Horta,Ana Cecília Góes (1) Miranda,Raquel Lima (1) Moreira,Renato de Azevedo (1) Ribeiro,Alexsandra Feitosa (1) Teixeira-Sá,Daniele Maria Alves (1) Mais... Palavra-chave Affinity chromatography (2) Cat liver lectin (1) Galactomannans (1) Galactose binding lectins (1) Galectins (1) Lectin (1) SS-Galactoside-binding lectin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction BABT Braga,Renata Chastinet; Teixeira-Sá,Daniele Maria Alves; Ribeiro,Alexsandra Feitosa; Miranda,Raquel Lima; Almeida,Lia Magalhães de; Horta,Ana Cecília Góes; Moreira,Renato de Azevedo. Lectins are proteins or glycoproteins able to bind, specifically and reversibly carbohydrates and glycoconjugates. Considering this ability, the utilization of Caesalpinia pulcherrima seeds polysaccharides as an affinity matrix was tested. The endospermic gum were solubilized in distinct concentrations of NaOH and treated with different amounts of epichlorohydrin (ECH) forming affinity gels with variable capacity for interaction with galactose- binding lectins. The gel with an ECH/gum ration of 6.0mmol/g was selected as the best affinity matrix. The matrix presented different efficiencies in terms of isolating galactose-binding lectins. C. pulcherrima endospermic galactomannans were purified by ethanol precipitation and the purified galactomannan was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Galactose binding lectins; Affinity chromatography; Galactomannans. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200009 Isolation of a ß-galactoside-binding lectin from cat liver BJMBR Franco-Fraguas,L.; Batista-Viera,F.; Carlsson,J.. A lectin from cat liver has been identified and purified by affinity chromatography on asialofetuin-Sepharose. One hundred micrograms of lectin was obtained from one cat liver with a purification factor of 1561. The lectin agglutinates trypsin-treated rabbit and cow erythrocytes. Hemagglutination was inhibited only by saccharides containing ß-galactosyl residues, of which the 1-amine-1-deoxy-ß-D-galactose was the most potent one by inhibiting hemagglutination at a concentration of 12.5 mM, followed by melibiose, trehalose and galactose. The lectin has a subunit molecular mass of 14.4 kDa determined by SDS-PAGE under reducing conditions and a pI of 4.85. Compared with the composition of lectins from calf heart and porcine heart, cat liver lectin contains... Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-Galactoside-binding lectin; Galectins; Lectin; Cat liver lectin; Affinity chromatography. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400005 Registros recuperados: 2 Primeira ... 1 ... Última

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