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| Registros recuperados: 55 | |
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| Marques, Odair José; UEM; Vidigal Filho, Pedro Soares; UEM; Dalpasquale, Valdecir Antoninho; UEM; Scapim, Carlos Alberto; UEM; Pricinotto, Luiz Fernando; UEM; Machinski Júnior, Miguel; UEM. |
| O objetivo deste trabalho foi avaliar a incidência de fungos dos gêneros Aspergillus, Fusarium e Penicillium e as contaminações com micotoxinas em grãos de cinco híbridos comerciais de milho em função da umidade de colheita. O trabalho foi conduzido em Astorga, Estado do Paraná, durante o período da ‘safrinha’ de 2007 e da safra de ‘verão’ de 2007/2008. As amostras de grãos, colhidas em cinco épocas distintas e em cinco repetições, foram submetidas à determinação de umidade pelo método da estufa a 103 ± 1ºC por 72h. O teste de sanidade Blotter test e a quantificação micotoxinas (método da cromatografia em camada delgada) foram efetuados, utilizando-se um delineamento inteiramente casualizado. Os resultados obtidos foram submetidos à análise de... |
| Tipo: Pesquisa Empírica de Campo |
Palavras-chave: 5.01.03.04-0 Produção de Mudas Zea mays L.; Pós-colheita; Qualidade; Aflatoxinas; Zearalenona Fitotecnia Zea mays L.; Post-harvest; Quality; Aflatoxins; Zearalenone. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5690 |
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| Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko. |
| In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025 |
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| MARTINS,Hermínia Marina; MARTINS,Maria Lígia; BERNARDO,Fernando Almeida. |
| Aflatoxins production is affected by abiotic, biotic and genetic parameters. Eugenesic and dysgenetic condition for aflatoxinogenesis are relatively well studied, such as: the influence of temperature, pH, Aw, Oxigen tension, osmotic pression, organic and inorganic nutrients, substance with fungistatic effects: however there are relatively few knowledge about endogenous regulator mechanisms, the kinetic of the anabolism and the interaction between the productive moulds and remaining microflora holding in eutrophying substrates. The main objective of this study is to evaluate biotic interaction between five indigenous strains of A. flavus, confirmed non-toxigenic, and a productive strain (A. parasiticus ATCC 15 517), cultured simultaneously on two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant interaction; Aflatoxins; Vegetal production; Aspergillus flavus; Aspergillus parasiticus. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600003 |
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| Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos. |
| Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007 |
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| Gonçalez,E.; Felicio,J.D.; Pinto,M.M.. |
| The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 µg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 µg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Ouratea sp; Aspergillus flavus; Biflavonoids; Aflatoxins. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100013 |
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| Gagini,Thalita B.; Silva,Robson E.; Castro,Isabela S.; Soares,Breno A.; Lima,Marco E.F.; Brito,Marilene F.; Mazur,Carlos; Direito,Glória M.; Danelli,Maria das Graças M.. |
| Aflatoxins are mycotoxins that have important toxic effects on human and animal health, even if consumed at low doses. The oral administration of piperine (1.12 mg/kg) during 23 days in rats seemingly interfered with the toxicity of aflatoxins, decreasing hepatic injuries and the leukocyte depletion in experimentally intoxicated animals. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Piperine; Experimental intoxication; Rats. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200013 |
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| Piedade,Fabiana Segatti; Fonseca,Homero; Gloria,Eduardo Micotti da; Calori-Domingues,Maria Antonia; Piedade,Sônia Maria Stefano; Barbin,Décio. |
| The aflatoxin distribution in corn fractions obtained after visual segregation for defects in 30 samples, known to be contaminated, was studied. Each sample was passed through a 5.0 mm round holes sieve, graded for defects and then segregated in sound kernels (regular kernels) and non-sound kernels (injured, germinated, fermented, moldy, heated, insect damaged, immature, broken, hollow, fermented up to ¼, discolored, extraneous materials, and injured by other causes), as defined by the Brazilian Official Grading rules for corn. The non-sound kernels showed the highest contamination levels in all samples. The contamination levels of non-sound kernels (20% of total weight) ranged from 23 to 1,365 µg/kg of aflatoxins (B1, B2, G1 and G2) and were higher than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Corn; Contamination distribution; Visual segregation; Fraction. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300013 |
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| Almeida,Adriana P.; Corrêa,Benedito; Mallozzi,Marisa A. B.; Sawazaki,Eduardo; Soares,Lúcia M. Valente. |
| The mycoflora of 3 hybrids of freshly harvested corn grains collected from three regions of the state of São Paulo, Brazil (Assis, Capão Bonito and Ribeirão Preto) was investigated. A total of 66 samples were analyzed focusing on the influence of abiotic factors (moisture content, water activity, temperature and rainfall) on both the prevalence of Aspergillus flavus and Fusarium moniliforme, and the ability of these genera isolates to produce aflatoxins and fumonisins, respectively. In the three surveyed regions, the fungal population comprised mainly Fusarium spp., Penicillium spp., Aspergillus spp. and 2 others filamentous fungal genera, which were isolated from corn kernels showing water activity of 0.30 to 0.99 and moisture content of 5.0% to 20.2%.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Aspergillus flavus; Fumonisins; Fusarium moniliforme; Corn. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400016 |
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| Giolo,Marcel Padovani; Oliveira,Christiane Minervino de; Bertolini,Dennis Armando; Lonardoni,Maria Valdrinez Campana; Gouveia,Matheus Sampaio; Netto,Daisy Pontes; Nixdorf,Suzana Lucy; Machinski Junior,Miguel. |
| Exposure to aflatoxins (AFs) in the diet may favour the development of hepatocellular carcinoma (HCC) and the acute exacerbation of hepatitis in chronic hepatitis B virus (HBV) carriers. Measurement of biomarkers such as aflatoxin M1 (AFM1), a metabolite of aflatoxin B1 (AFB1), in urine allows for the assessment of populations exposed to aflatoxins. The aim of this study was to investigate the occurrence of aflatoxin M1 in the urine of HBV carrier and non-carrier patients. One group included 43 randomly selected HBV carriers treated at two hospitals in the city of Maringa, Brazil, from March to June 2008. Control group consisted of 29 healthy adult volunteers with anti-HBs positive and HBsAg negative test results. Detection of AFM1 was performed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Biomarkers; Hepatitis B; Hepatocellular carcinoma; Urina/análise toxicológica; Epidemiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300011 |
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| Chalfoun,Sára Maria; Pereira,Marcelo Cláudio; Resende,Mario Lúcio V.; Angélico,Caroline Lima; Silva,Rozane Aparecida da. |
| The effect of ten powdered spice plants was evaluated at the concentration of 1, 2, 3 and 4% to observe the mycelial growth and sporulation of Aspergillus niger and Eurotium repens. The spices were added to the culture media PDA and CYA20S. Clove completely inhibited the mycelial growth of the tested fungi. The other spices: cinnamon, garlic, thyme, mint, anis, oregano and onion were, in a decreasing order, promising antifungals. Bay leaf and basil did not show a pronounced fungistatic effect. The antitoxigenic potential of the spices was tested against one aflatoxin-producing strain of AspergiIIus flavus. The spices were tested at the same concentrations previously mentioned and were added to the culture medium YES, appropriate for the production of those... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spices; Aflatoxins; Control; Fungi. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000400018 |
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| FARIA,Carla Bertechini; SANTOS,Fabiane Cristina dos; CASTRO,Fausto Fernandes de; SUTIL,Ariadne Ricieli; SERGIO,Luciana Marciano; SILVA,Milena Veronezi; MACHINSKI JUNIOR,Miguel; BARBOSA-TESSMANN,Ione Parra. |
| Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus flavus; Aflatoxins; Bulgur wheat; PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103 |
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| Mariutti,Lilian Regina Barros; Soares,Lucia Maria Valente. |
| Tomatoes are highly susceptible to fungi contamination in the field, during transportation, processing, and storage. Aspergillus flavus and Aspergillus parasiticus have been isolated from tomatoes and tomato products, and both fungi species can produce aflatoxin, mycotoxin with hepatotoxic, carcinogenic, teratogenic, and mutagenic effects on all animal species tested so far. In order to verify a possible aflatoxin contamination of tomato products commercialized in Brazil, 63 samples of tomato products (pulp, paste, purée, ketchup, dehydrated tomatoes, and dried tomatoes preserved in oil) produced in 5 Brazilian states and 1 imported sample (ketchup), totalizing 29 brands, were analyzed by thin layer chromatography. The analytical method showed an average... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycotoxins; Aflatoxins; Tomato products. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000200031 |
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| CASTRO,Luciana de; VARGAS,Eugenia Azevedo. |
| A method for determining aflatoxins B1 (AFB1), B2 (AFB2),G1 (AFG1) andG2 (AFG2) in maize with florisil clean up was optimised aiming at one-dimensional thin layer chromatography (TLC) analysis with visual and densitometric quantification. Aflatoxins were extracted with chloroform: water (30:1, v/v), purified through florisil cartridges, separated on TLC plate, detected and quantified by visual and densitometric analysis. The in-house method performance characteristics were determined by using spiked, naturally contaminated maize samples, and certified reference material. The mean recoveries for aflatoxins were 94.2, 81.9, 93.5 and 97.3% in the range of 1.0 to 242 µg/kg for AFB1, 0.3 to 85mg/kg for AFB2, 0.6 to 148mg/kg for AFG1 and 0.6 to 140mg/kg for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Florisil; Maize; Thin-layer chromatography (TLC); Densitometry. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000100024 |
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| VILLEGAS-RASCÓN,Rocio Elizabeth; LÓPEZ-MENESES,Ana Karenth; PLASCENCIA-JATOMEA,Maribel; COTA-ARRIOLA,Octavio; MORENO-IBARRA,Griselda Macrina; CASTILLÓN-CAMPAÑA,Lucía Guadalupe; SÁNCHEZ-MARIÑEZ,Reyna Isabel; CORTEZ-ROCHA,Mario Onofre. |
| Abstract Aspergillus and Fusarium are the fungi genera most frequently isolated from cereal grains and other commodities. They are capable of producing mycotoxins, which can affect the human and animal health. Synthetic fungicides have been used to control these fungi, nevertheless, they have acquired resistance and other alternatives are necessary since they now need higher amounts. Therefore, the aim of this study was to evaluate the efficacy of cinnamon, clove and thyme essential oils (EOs) alone and encapsulated in chitosan on the radial growth, spore germination and mycotoxin production by Fusarium verticillioides and Aspergillus parasiticus. The composition of the EOs was determined by gas chromatography mass spectrometry (GC-MS). EOs inhibited... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Fumonisins; Radial growth; Spore germination. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000200335 |
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| GONÇALVES,Luana; DALLA ROSA,Andréia; GONZALES,Samantha Lemke; FELTES,Maria Manuela Camino; BADIALE-FURLONG,Eliana; DORS,Giniani Carla. |
| Abstract The objective of this work was to determine aflatoxin M1 in fresh milk from fifty-two small farms in the city of Concórdia - SC, Brazil. Samples from the cooling tanks of each property were collected from November 2014 to January 2015. The QuEChERS method was used for the extraction of aflatoxin M1, and quantification was performed in UHPLC-FL. 40.4% of the analyzed samples (eg, 21 samples) showed contamination levels by aflatoxin M1 above the maximum limit allowed by the Brazilian regulation, which is 0.5 μg L-1. These results suggest the importance of implementing Good Practices in obtaining feed for dairy cows, since the contamination of milk by aflatoxin M1 occurs through the biotransformation of aflatoxin B1, after the ingestion of feed or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; QuEChERS; UHPLC-FL. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000700011 |
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| Santos,Alini Cristini dos; Souza,Aline Amenencia de; Silva,Milena Veronezi; Nerilo,Samuel Botião; Souza,Alexandra Perdigão Maia de; Bando,Érika; Machinski Junior,Miguel. |
| ABSTRACT: Peanuts are a nutritious food consumed worldwide. Some species of the genus Aspergillus, producers of aflatoxins, colonize peanuts. Human exposure to aflatoxins occurs by ingesting contaminated foods. The objective of this paper was to assess the occurrence and dietary exposure of the population to aflatoxins in peanuts commercially available in Maringa, Brazil, from May 2013 to April 2017. A total of 104 peanut samples were evaluated by thin-layer chromatography and confirmed by derivatization with trifluoroacetic acid. The contamination rate detected was 24.0% in average concentration of 13.4µg/kg. Twenty positive samples (19.2%) exceeded the maximum level permitted in Brazil for the sum of four aflatoxins. Estimated probable daily intake was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Aspergillus flavus; Peanuts; Risk assessment; Public health. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600750 |
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| GONCALVES, R. C.; ALVARES, V. de S.; CARTAXO, C. B. da C.; WADT, L. H. de O.; SOUZA, J. M. L. de; LIMA, A. C. de; COSTA, D. A. da; GIACOMELLI, M.; MAGALHÃES, K. da S.; MADRUGA, A. L. S.. |
| Com o objetivo de disponibilizar uma tecnologia propícia para a secagem de sementes no local de coleta que tiver energia hidroelétrica ou por motor estacionário, foi estudada na Embrapa Acre a eficiência de três secadores de sementes de castanheira-da-amazônia, sendo dois por convecção natural de ar à sombra e ao sol, respectivamente, e um secador a ar aquecido forçado artificialmente (SAAA). |
| Tipo: Folhetos |
Palavras-chave: Castanha do brasil; Secador estacionário; Cáscaras de fruta; Temperatura de secado; Secadores de convección; Nuez del Brasil; Micotoxinas; Secagem natural; Aflatoxinas; Calidad de la fruta; Castanha do pará; Secagem artificial; Resistência a temperatura; Contaminação fúngica; Micotoxina; Aflatoxina; Bertholletia excelsa; Fruto; Qualidade; Casca; Propriedade físico-química; Aspergillus flavus; Fruit quality; Brazil nuts; Mycotoxins; Convection dryers; Drying temperature; Fungi; Aflatoxins; Fruit peels. |
| Ano: 2010 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/872684 |
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| CARTAXO, C. B. da C.; SUASSUNA, T. de M. F.; GONDIM, T. M. de S.. |
| O amendoim é uma cultura oleaginosa que constitui importante fonte de óleos e proteínas para populações do Mali e do Brasil. Em ambos os países, é produzido, principalmente, por pequenos produtores, em áreas que variam de 0,3 ha a 5 ha, com espaçamentos entrelinhas de, aproximadamente, 0,50 m, utilizando-se de 10 a 20 sementes por metro e gastando-se em torno de 90 kg de sementes/ha. A contaminação por aflatoxinas é o principal problema associado à cultura e está intimamente ligada ao crescimento de fungos (bolores) que se desenvolvem, favorecidos pelas condições climáticas nos dois países. |
| Tipo: Folders |
Palavras-chave: Boas práticas; Brasil; Aflatoxinas; Crecimiento microbiano; Prevención de enfermedades; Amendoim; Arachis hypogaea; Produção; Contaminação fúngica; Aflatoxina; Microbial growth; Aflatoxins; Disease prevention; Mali. |
| Ano: 2017 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086125 |
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| Registros recuperados: 55 | |
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