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	Provedor de dados BJPS (2) BJM (1) Autor Corrêa,Marcos Antonio (1) Fiorentino,Flávia Angélica Másquio (1) Leonel,Míriam de Fátima Vianna (1) Nunan,Elzíria de Aguiar (1) Pereira,Larissa de Melo Campos Sousa (1) Salgado,Hérida Regina Nunes (1) Smânia Jr.,Artur (1) Smânia,Elza F A (1) Solano,Ana Gabriela Reis (1) Souza,Simone Machado de (1) Valgas,Cleidson (1) Mais... Palavra-chave Agar diffusion (3) Antimicrobial activity (1) Antiseptic (1) Bioautographic methods (1) Chlorhexidine (1) Cylinder-plate (1) Gramicidin/microbiological assay (1) Microbiological assay (1) Microbiological assay/method validation (1) Minimum bactericidal concentration (1) Minimum inhibitory concentration (1) Natural products (1) Quality control (1) Validation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2013 (1) 2011 (1) 2007 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Screening methods to determine antibacterial activity of natural products BJM Valgas,Cleidson; Souza,Simone Machado de; Smânia,Elza F A; Smânia Jr.,Artur. The emergence of new infectious diseases, the resurgence of several infections that appeared to have been controlled and the increase in bacterial resistance have created the necessity for studies directed towards the development of new antimicrobials. Considering the failure to acquire new molecules with antimicrobial properties from microorganisms, the optimization for screening methods used for the identification of antimicrobials from other natural sources is of great importance. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar diffusion; Antimicrobial activity; Bioautographic methods; Minimum inhibitory concentration; Minimum bactericidal concentration; Natural products. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200034 Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution BJPS Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes. Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017 Development of agar diffusion method for dosage of gramicidin BJPS Solano,Ana Gabriela Reis; Pereira,Larissa de Melo Campos Sousa; Leonel,Míriam de Fátima Vianna; Nunan,Elzíria de Aguiar. Gramicidin, an antimicrobial peptide active against Gram positive bacteria, is commonly used in pharmaceutical preparations for topical use. Considering that only the turbidimetric method has been described in the literature, the present study sought to develop and validate an agar diffusion method for the dosage of gramicidin. The method was developed and validated using the Kocuria rhizophila ATCC 9341 as a test microorganism. Two designs were used: a 3x3 parallel-line model, and a 5x1 standard curve. The validation demonstrated that the method follows the linear model (r²= 0.994), presenting a significant regression between the zone diameter of growth inhibition and the logarithm of the concentration within the range of 5 to 25.3 µg/mL. The results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gramicidin/microbiological assay; Microbiological assay/method validation; Agar diffusion; Cylinder-plate. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300014 Registros recuperados: 3 Primeira ... 1 ... Última

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