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| Xiao,Na; Tang,Yi-Tong; Li,Zhi-Shan; Cao,Rui; Wang,Rong; Zou,Jiu-Ming; Pei,Jiao. |
| Abstract This study aimed to develop a simple and rapid method to detect KRAS gene mutations for conventional clinical applications under laboratory conditions. The genotype of mutation sites was determined based on the occurrence of target bands in the corresponding lanes of the reaction tubes through polymerization-conjunction of the probes, probe purification and amplification, and agarose gel electrophoresis. Circulating DNA samples were obtained from the plasma of 72 patients with lung cancer, which were identified based on six mutation sites (G12S, G12R, G12C, G12D, G12A, and G12V) of codon 12 of the KRAS gene. The detection results were compared with direct sequencing data. The proposed detection method is characterized by simple operation, high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mutation; Circulating DNA; Polymerization-conjunction reaction; Agarose gel electrophoresis; K-ras. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400555 |
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| Okatsu, Y.; Yamagishi, N.; Hatate, K.; Devkota, B.. |
| The aim of this study was to show the usefulness of a commercial agarose gel electrophoresis (AGE) kit (QuickGel SP) for separating bovine serum protein fractions in comparison with conventional cellulose acetate electrophoresis (CAE). Serum protein bands were verified using five reference reagents corresponding to albumin and α1-, β1-, β2-, and γ-globulins. AGE clearly revealed six separated fractions of albumin and α1-, α2-, β1-, β2-, and γ-globulin fractions in 100% and 77.8% in serum samples of dairy cows from the healthy (n=27) and diseased groups (n=27), respectively. The α1- and α2-globulins were not separated by CAE in 14.8% and 96.3% of the samples from the healthy and diseased groups, respectively, whereas β2- and γ-globulin were not separated by... |
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Palavras-chave: Agarose gel electrophoresis; Bovine serum protein; Cellulose acetate electrophoresis; QuickGel SP. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4580 |
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