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| Gay, Melanie; Berthe, Franck; Leroux, Frederique. |
| In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials under laboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screening revealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C, gigas mortality rates in experimental challenges. Although the study... |
| Tipo: Text |
Palavras-chave: Crassostrea gias; Antagonism; Agonism; Virulence; Vibrio splendidus. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-3174.pdf |
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| Gay, Melanie; Renault, Tristan; Pons, Anne-marie; Le Roux, Frédérique. |
| For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor... |
| Tipo: Text |
Palavras-chave: Histopathology; Molecular taxonomy; Agonism; Vibriosis. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2912.pdf |
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