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| Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F.. |
| The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002 |
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| Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Kumar,Vinod; Lakshmanan,Venkatachalam; Venkataramareddy,Sreedhar Reddampalli; Aswathanarayana,Ravishankar Gokare. |
| The genetically transformed roots of red beet have been shown, for the first time, to produce very high levels of peroxidase (POD; EC 1.11.1.7) accounting for 1.21 x 10(6) Units L-1. Of the ten clones established using different strains of Agrobacterium rhizogenes, one was that from the strain LMG-150, three each from A 2/83, A 20/83 and A4. All the clones showed true integration of T-DNA when tested by PCR and Southern hybridization methods. Each clone differed significantly from the others in growth, hormone dependency and POD production where LMG-150 produced highest biomass (140 g FW L-1) as well as POD (ranging from 8000-9000 U g-1 FW and 1.18 x 10(6) U L-1 with a specific activity of 600 U mg-1 protein) on hormone-free medium, both in shake-flask as... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium rhizogenes; Auxins; Elicitation; T-DNA; Thermostability; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200008 |
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| BARROS, L. M. G.; ARRIAL, R. T.; ACAUÃ, T.; ALMEIDA, J. D.; CARNEIRO, M.. |
| A proteina RolA e originaria de Agrobacterium rhizogenes, uma bacteria fitopatogenica que provoca a doenca conhecida como raiz em cabeleira (?ghairy root?h). RolA atua no processo de infeccao, sendo codificada no T-DNA do plasmideo Ri da agrobacteria. Embora a expressao de RolA em plantas implique em severas alteracoes morfologicas e fisiologicas, seu mecanismo de acao e desconhecido. A identificacao da regiao de RolA responsavel pela sua funcao e sua localizacao subcelular sao dados importantes na elucidacao de seu papel biologico. Com o objetivo de abordar esta questao, foram feitas fusoes traducionais da regiao codificadora de RolA completa e truncada com a regiao codificadora da enzima ?À-glucuronidase, sob regulacao do promotor CaMV35S. Plantas de... |
| Tipo: Folhetos |
Palavras-chave: Proteína RolA; Agrobacterium rhizogenes; Região amino-terminal; Atividade biológica. |
| Ano: 2005 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187221 |
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