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Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles Rev. Bras. Frutic.
Brancher,Thyana Lays; Hawerroth,Maraisa Crestani; Hawerroth,Fernando José; Kvitschal,Marcus Vinícius; Denardi,Frederico; Guidolin,Altamir Frederico.
Abstract The objective of this study was to characterize the parents and respective populations of apple trees regarding S-alleles to confirm their genealogy and to evaluate the efficiency of the molecular markers used. Sixteen specific sets of primers were used for identification of apple S-alleles by PCR. Two segregating populations of the Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ and ‘M-11/00’ × ‘M-13/91’ were evaluated. The expected segregations are 1:1:1:1 for full compatibility and 1:1 for semi-compatibility, which can be confirmed by the X2 test. The ‘Fred Hough’ (S5S19) × ‘Monalisa’ (S2S10) cross proved to be fully compatible; and two triploids were identified among the hybrids as well. The ‘M-11/00’...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malus × domestica Borkh; S genotype; S-RNase; Allele-specific PCR; Segregation.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452021000100801
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