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| Soares,Maria Amélia Menck; Rodrigues,Marcelo Teixeira; Mognol,Giuliana Patrícia; Ribeiro,Lucinéia de Fátima Chasko; Silva,José Luis da Conceição; Brancalhão,Rose Meire Costa. |
| Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine the frequencies of major alleles for null, low, intermediate and high milk protein expression in a herd of dairy goats raised in the southeastern region of Brazil. Genomic DNA samples were obtained from leukocytes of 145 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in both agarose (O and E allele) and polyacrylamide gels (F allele). For better characterization of the F allele, a PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) study was performed employing the endonuclease XmnI. The allelic frequencies in the herd of 62 Saanen goats studied were: CSN1S1E = 0.35; CSN1S1F = 0.30; CSN1S1O1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpine; Capra hircus; CSN1S1; Genotyping; Milk proteins; Saanen. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600008 |
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